Overview

  • Product name
  • Description
    Rabbit polyclonal to Nicastrin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Nicastrin (internal sequence). (18 amino acid peptide; NP_056146.1).
    Database link: Q92542

  • Positive control
    • Human skin tissue; Human brain cells; Human brain lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab189125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 78 kDa.

Target

  • Function
    Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the nicastrin family.
  • Cellular localization
    Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Anterior pharynx defective 2 antibody
    • APH 2 antibody
    • APH2 antibody
    • ATAG1874 antibody
    • KIAA0253 antibody
    • Ncstn antibody
    • NCT antibody
    • NICA_HUMAN antibody
    • Nicastrin antibody
    • RP11 517F10.1 antibody
    • RP11517F101 antibody
    see all

Images

  • Immunofluorescent analysis of Human brain cells labeling Nicastrin with ab189125 at 20 µg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue labeling Nicastrin with ab189125 at 5 µg/ml.

  • Lane 1 : Anti-Nicastrin antibody (ab189125) at 0.5 µg/ml
    Lane 2 : Anti-Nicastrin antibody (ab189125) at 1 µg/ml

    All lanes : Human brain lysate

    Predicted band size: 78 kDa

References

ab189125 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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