|
|
|
|
Immunohistochemistry (Paraffin-embedded sections) - Nicotinic Acetylcholine Receptor alpha antibody (ab28489)
(enlarge)
|
|
Western blot - Nicotinic Acetylcholine Receptor alpha antibody (ab28489)
(enlarge)
|
|
|
|
|
Product Name
|
Nicotinic Acetylcholine Receptor alpha 1 antibody
|
| |
See all Nicotinic Acetylcholine Receptor alpha 1 antibodies (3)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 1
|
|
Immunogen
|
Synthetic peptide derived from residues 349 - 398 of human Nicotinic Acetylcholine Receptor alpha.
.
|
|
Reacts with
(species key)
|
Hu
|
|
Specificity
|
ab28489 recognises Nicotinic Acetylcholine Receptor alpha one isoform a and b (muscle isoforms).
|
|
Tested applications
(see key)
|
ELISA, IHC-P, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ELISA: 1/62500.
IHC-P: Use at a concentration of 4 - 8 µg/ml.
WB: Use at a concentration of 0.12 µg/ml. Predicted molecular weight: 54 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Human intestine, fetal heart lysate.
|
|
Cellular localization
|
Cell Membrane
|
|
Research areas
|
Neuroscience >> Neurotransmission >> Receptors / Channels >> Ligand-Gated Ion Channels >> nAch Receptors
|
|
Relevance
|
After binding acetylcholine, Nicotinic Acetylcholine Receptor alpha 1 responds by an extensive change in conformation that affects all subunits and leads to opening of an ion conducting channel across the plasma membrane. Isoform 1 is only expressed in skeletal muscle whereas isoform 2 is constitutively expressed in skeletal muscle, brain, heart, kidney, liver, lung and thymus
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: None
Constituents: 2% Sucrose, PBS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Nicotinic Acetylcholine Receptor alpha 1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for Nicotinic Acetylcholine Receptor alpha 1 antibody images, references, products related to ab28489 and other tools.
|
| |
Nicotinic Acetylcholine Receptor alpha 1 antibody images:
|
 Immunohistochemistry (Paraffin-embedded sections) - Nicotinic Acetylcholine Receptor alpha antibody (ab28489)
Immunohistochemical analysis of Nicotinic Acetylcholine Receptor alpha expression in paraffin embedded human intestine tissue section using 4 µg/ml ab28489. Arrows indicate positively labelled epithelial cells of intestinal villus.
|
| |
Western blot - Nicotinic Acetylcholine Receptor alpha antibody (ab28489)
Nicotinic Acetylcholine Receptor alpha 1 antibody (ab28489) at 0.12 µg/ml + Fetal heart lysate
Secondary
HRP conjugated anti-Rabbit IgG HRP conjugated anti-Rabbit IgG (diluted in 1: 50,000 - 100,000)
Predicted band size : 54 kDa
|
| |
| |
|
Search PubMed (MEDLINE) for references to Nicotinic Acetylcholine Receptor alpha 1
|
| |
Nicotinic Acetylcholine Receptor alpha 1 antibody - more information
|
Customer reviews (feedback) regarding Nicotinic Acetylcholine Receptor alpha 1 antibody |
Customer FAQs regarding Nicotinic Acetylcholine Receptor alpha 1 antibody |
Protocols for Nicotinic Acetylcholine Receptor alpha 1 antibody |
Price and availability of products related to Nicotinic Acetylcholine Receptor alpha 1 antibody |
| |
Nicotinic Acetylcholine Receptor alpha 1 antibody - related products:
|
|
|
| |
Nicotinic Acetylcholine Receptor alpha 1 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about Nicotinic Acetylcholine Receptor alpha 1 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
