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Immunohistochemistry (Paraffin-embedded sections) - Nicotinic Acetylcholine Receptor alpha antibody (ab28489)

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Western blot - Nicotinic Acetylcholine Receptor alpha antibody (ab28489)

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Product Name
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Nicotinic Acetylcholine Receptor alpha 1 antibody
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See all Nicotinic Acetylcholine Receptor alpha 1 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 1
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Immunogen
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Synthetic peptide derived from residues 349 - 398 of human Nicotinic Acetylcholine Receptor alpha.
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Reacts with
(species key)
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Hu
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Specificity
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ab28489 recognises Nicotinic Acetylcholine Receptor alpha one isoform a and b (muscle isoforms).
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions ELISA: 1/62500. IHC-P: Use at a concentration of 4 - 8 µg/ml. WB: Use at a concentration of 0.12 µg/ml. Predicted molecular weight: 54 kDa.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human intestine, fetal heart lysate.
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Cellular localization
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Cell Membrane
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Research areas
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Neuroscience >> Neurotransmission >> Receptors / Channels >> Ligand-Gated Ion Channels >> nAch Receptors
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Relevance
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After binding acetylcholine, Nicotinic Acetylcholine Receptor alpha 1 responds by an extensive change in conformation that affects all subunits and leads to opening of an ion conducting channel across the plasma membrane. Isoform 1 is only expressed in skeletal muscle whereas isoform 2 is constitutively expressed in skeletal muscle, brain, heart, kidney, liver, lung and thymus
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Nicotinic Acetylcholine Receptor alpha 1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Nicotinic Acetylcholine Receptor alpha 1 antibody images, references, products related to ab28489 and other tools.
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Nicotinic Acetylcholine Receptor alpha 1 antibody images:
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Immunohistochemistry (Paraffin-embedded sections) - Nicotinic Acetylcholine Receptor alpha antibody (ab28489)
Immunohistochemical analysis of Nicotinic Acetylcholine Receptor alpha expression in paraffin embedded human intestine tissue section using 4 µg/ml ab28489. Arrows indicate positively labelled epithelial cells of intestinal villus.
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Western blot - Nicotinic Acetylcholine Receptor alpha antibody (ab28489)
Nicotinic Acetylcholine Receptor alpha 1 antibody (ab28489) at 0.12 µg/ml + Fetal heart lysate
Secondary HRP conjugated anti-Rabbit IgG HRP conjugated anti-Rabbit IgG (diluted in 1: 50,000 - 100,000)
Predicted band size : 54 kDa
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Search PubMed (MEDLINE) for references to Nicotinic Acetylcholine Receptor alpha 1
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Nicotinic Acetylcholine Receptor alpha 1 antibody - more information
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Customer reviews (feedback) regarding Nicotinic Acetylcholine Receptor alpha 1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"