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Anti-Nicotinic Acetylcholine Receptor alpha 1 antibody (ab28489)

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Overview

Product name

Anti-Nicotinic Acetylcholine Receptor alpha 1 antibody
See all Nicotinic Acetylcholine Receptor alpha 1 products (3) ...

Description

Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 1

Specificity

ab28489 recognises Nicotinic Acetylcholine Receptor alpha one isoform a and b (muscle isoforms).

Tested applications

WB, IHC-P, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Chicken, Cow, Dog

Immunogen

Synthetic peptide derived from residues 349 - 398 of human Nicotinic Acetylcholine Receptor alpha.

.

Positive control

Human intestine, fetal heart lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 2% Sucrose, PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Immunohistochemistry (Paraffin-embedded sections) - Nicotinic Acetylcholine Receptor alpha antibody (ab28489)Immunohistochemistry (Paraffin-embedded sections) - Nicotinic Acetylcholine Receptor alpha antibody (ab28489) image (enlarge)

  • Western blot - Nicotinic Acetylcholine Receptor alpha antibody (ab28489)Western blot - Nicotinic Acetylcholine Receptor alpha antibody (ab28489) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab28489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

Tissue specificity

Isoform 1 is only expressed in skeletal muscle. Isoform 2 is constitutively expressed in skeletal muscle, brain, heart, kidney, liver, lung and thymus.

Involvement in disease

Defects in CHRNA1 are a cause of multiple pterygium syndrome lethal type (MUPSL) [MIM:253290]. Multiple pterygia are found infrequently in children with arthrogryposis and in fetuses with fetal akinesia syndrome. In lethal multiple pterygium syndrome there is intrauterine growth retardation, multiple pterygia, and flexion contractures causing severe arthrogryposis and fetal akinesia. Subcutaneous edema can be severe, causing fetal hydrops with cystic hygroma and lung hypoplasia. Oligohydramnios and facial anomalies are frequent.
Note=The alpha subunit is the main focus for antibody binding in myasthenia gravis. Myasthenia gravis is characterized by sporadic muscular fatigability and weakness, occurring chiefly in muscles innervated by cranial nerves, and characteristically improved by cholinesterase-inhibiting drugs.
Defects in CHRNA1 are a cause of congenital myasthenic syndrome slow-channel type (SCCMS) [MIM:601462]. SCCMS is the most common congenital myasthenic syndrome. Congenital myasthenic syndromes are characterized by muscle weakness affecting the axial and limb muscles (with hypotonia in early-onset forms), the ocular muscles (leading to ptosis and ophthalmoplegia), and the facial and bulbar musculature (affecting sucking and swallowing, and leading to dysphonia). The symptoms fluctuate and worsen with physical effort. SCCMS is caused by kinetic abnormalities of the AChR, resulting in prolonged endplate currents and prolonged AChR channel opening episodes.
Defects in CHRNA1 are a cause of congenital myasthenic syndrome fast-channel type (FCCMS) [MIM:608930]. FCCMS is a congenital myasthenic syndrome characterized by kinetic abnormalities of the AChR. In most cases, FCCMS is due to mutations that decrease activity of the AChR by slowing the rate of opening of the receptor channel, speeding the rate of closure of the channel, or decreasing the number of openings of the channel during ACh occupancy. The result is failure to achieve threshold depolarization of the endplate and consequent failure to fire an action potential.

Sequence similarities

Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-1/CHRNA1 sub-subfamily.

Cellular localization

Cell junction > synapse > postsynaptic cell membrane. Cell membrane.

Target information above from: UniProt accessionP02708 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Acetylcholine receptor protein alpha chain Precursor antibody
  • Acetylcholine receptor protein alpha chain Precursor antibody
  • Acetylcholine receptor subunit alpha antibody
  • ACHA_HUMAN antibody
  • ACHRA antibody
  • ACHRA antibody
  • CHNRA antibody
  • CHNRA antibody
  • Cholinergic receptor nicotinic alpha polypeptide 1 antibody
  • Cholinergic receptor nicotinic alpha polypeptide 1 antibody
  • Cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle) antibody
  • CHRNA1 antibody
  • CHRNA1 antibody
  • Human mRNA for muscle acetylcholine receptor alpha subunit antibody
  • Human mRNA for muscle acetylcholine receptor alpha subunit antibody
  • Nicotinic cholinergic receptor alpha 1 antibody
  • Schizophrenia neurophysiologic defect candidate antibody
  • Schizophrenia neurophysiologic defect candidate antibody
see all

Anti-Nicotinic Acetylcholine Receptor alpha 1 antibody images:

  Immunohistochemistry (Paraffin-embedded sections) - Nicotinic Acetylcholine Receptor alpha antibody (ab28489)

Immunohistochemistry (Paraffin-embedded sections) - Nicotinic Acetylcholine Receptor alpha antibody (ab28489)

Immunohistochemical analysis of Nicotinic Acetylcholine Receptor alpha expression in paraffin embedded human intestine tissue section using 4 µg/ml ab28489. Arrows indicate positively labelled epithelial cells of intestinal villus.

  Western blot - Nicotinic Acetylcholine Receptor alpha antibody (ab28489)

Western blot - Nicotinic Acetylcholine Receptor alpha antibody (ab28489)

Anti-Nicotinic Acetylcholine Receptor alpha 1 antibody (ab28489) at 0.12 µg/ml + Fetal heart lysate

Secondary
HRP conjugated anti-Rabbit IgG HRP conjugated anti-Rabbit IgG (diluted in 1: 50,000 - 100,000)

Predicted band size : 54 kDa

References for Anti-Nicotinic Acetylcholine Receptor alpha 1 antibody (ab28489)

ab28489 has not yet been referenced specifically in any publications.

Publishing research using ab28489? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"