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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nicotinic Acetylcholine Receptor alpha 1 antibody (ab65832)
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Western blot - Nicotinic Acetylcholine Receptor alpha 1 antibody (ab65832)
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Product Name
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Nicotinic Acetylcholine Receptor alpha 1 antibody
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See all Nicotinic Acetylcholine Receptor alpha 1 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 1
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Immunogen
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Synthetic peptide (Human) corresponding to a sequence at the N terminus of Nicotinic Acetylcholine Receptor alpha 1, identical to the related mouse and rat sequence.
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Reacts with
(species key)
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Hu, Ms, Rat, Rb
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at a concentration of 1 - 2 µg/ml.
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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IHC-P: Rat brain tissue
WB: Rat brain lysate
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Cellular localization
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Cell Membrane
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Research areas
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Neuroscience >> Neurotransmission >> Receptors / Channels >> Ligand-Gated Ion Channels >> nAch Receptors
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Relevance
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After binding acetylcholine, Nicotinic Acetylcholine Receptor alpha 1 responds by an extensive change in conformation that affects all subunits and leads to opening of an ion conducting channel across the plasma membrane. Isoform 1 is only expressed in skeletal muscle whereas isoform 2 is constitutively expressed in skeletal muscle, brain, heart, kidney, liver, lung and thymus
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Nicotinic Acetylcholine Receptor alpha 1 antibody (ab65832) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide, 0.05mg Thimerosal
Constituents: 5mg BSA, 0.9mg Sodium chloride, 0.2mg Dibasic Sodium Phosphate Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Nicotinic Acetylcholine Receptor alpha 1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Nicotinic Acetylcholine Receptor alpha 1 antibody images, references, products related to ab65832 and other tools.
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Nicotinic Acetylcholine Receptor alpha 1 antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nicotinic Acetylcholine Receptor alpha 1 antibody (ab65832)
ab65832, at 2µg/ml, staining Nicotinic Acetylcholine Receptor alpha 1 in paraffin embedded rat brain tissue sections by Immunohistochemistry.
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Western blot - Nicotinic Acetylcholine Receptor alpha 1 antibody (ab65832)
Nicotinic Acetylcholine Receptor alpha 1 antibody (ab65832) at 2 µg/ml + rat brain lysate
Predicted band size : 54 kDa
Observed band size : 54 kDa
Additional bands at : 39 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to Nicotinic Acetylcholine Receptor alpha 1
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Nicotinic Acetylcholine Receptor alpha 1 antibody - more information
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Customer FAQs regarding Nicotinic Acetylcholine Receptor alpha 1 antibody |
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