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Product name
Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody
See all Nicotinic Acetylcholine Receptor alpha 7 products (5) ...
Description
Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 7
Specificity
Several significant splice variants of the protein have been reported, but currently, we do not know, which variants are detected by this antibody.
Tested applications
ICC/IF, IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Chicken, Cow, Cat, Dog
Immunogen
Synthetic peptide conjugated to KLH, selected from within the amino acid range 22-71 of Human Nicotinic Acetylcholine Receptor alpha 7. (Peptide available as ab10 1467.)
Positive control
HepG2 lysate and Mouse muscle lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, Sucrose
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolism processes >> Hypoxia
Cancer >> Cancer Metabolism >> Response to hypoxia
Neuroscience >> Neurotransmission >> Receptors / Channels >> Ligand-Gated Ion Channels >> nAch Receptors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10096 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
WB: Use a concentration of 2.5 - 5 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).Can be blocked with Nicotinic Acetylcholine Receptor alpha 7 peptide (ab101467). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Target
Function
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.
Sequence similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-7/CHRNA7 sub-subfamily.
Cellular localization
Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
Target information above from: UniProt accessionP36544
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- A7 nicotinic acetylcholine receptor antibody
- Acetylcholine receptor alpha 7 neural antibody
- ACHA7_HUMAN antibody
see all
Database links
- Entrez Gene: 374001 Chicken
- Entrez Gene: 282178 Cow
- Entrez Gene: 1139 Human
- Entrez Gene: 11441 Mouse
- Entrez Gene: 25302 Rat
- Omim: 118511 Human
- SwissProt: P22770 Chicken
- SwissProt: P54131 Cow
see all
Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody images:
Western blot - Nicotinic Acetylcholine Receptor alpha 7 antibody (ab10096)
Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab10096) at 2.5 µg/ml + HepG2 cell lysate
Predicted band size : 56 kDa
Western blot - Nicotinic Acetylcholine Receptor alpha 7 antibody (ab10096)
Predicted band size : 56 kDa
WB using ab10096, using two different blocking conditions. Best signal seen with 3% BSA and 10% FBS solution. Review by Andreas Wiesner submitted 7 May 2004 (see review section for further information).
Western blot - Nicotinic Acetylcholine Receptor alpha 7 antibody (ab10096)
Predicted band size : 56 kDa
WB using ab10096. Blots were incubated with ab10096 for at least 1 hour and for 30-90 minutes for the secondary antibody. 3% BSA and 10% FBS was used as blocking solution. anti- bungarotoxin was added in excess before precipitation in lanes 2 and 4. Nicotinic acetylcholine receptor alpha 7 precipitates as a double band (as shown by Drisdel and Green, 2000). The higher bands (79-122 kD) are probably dimeric and trimeric forms of the protein. Review by Andreas Wiesner submitted 7 May 2004.
References for Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab10096)
This product has been referenced in:
- Greene CM et al. Inhibition of Toll-like receptor 2-mediated interleukin-8 production in Cystic Fibrosis airway epithelial cells via the alpha7-nicotinic acetylcholine receptor. Mediators Inflamm 2010:423241 (2010). ICC/IF; Human.Read more (PubMed: 20379354) »
- Machaalani R et al. Distribution of nicotinic acetylcholine receptor subunits alpha7 and beta2 in the human brainstem and hippocampal formation. J Chem Neuroanat 40:223-31 (2010). IHC-P; Human.Read more (PubMed: 20566330) »
See all 7 publications for this product
Publishing research using ab10096? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"