Recombinant Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (ab124801)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5717] to Niemann Pick C1 Like 1/NPC1L1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717]
See all Niemann Pick C1 Like 1/NPC1L1 primary antibodies -
Description
Rabbit monoclonal [EPR5717] to Niemann Pick C1 Like 1/NPC1L1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal liver and HeLa lysates; Human liver tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5717 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa). |
Target
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Function
Play a major role in cholesterol homeostasis. Is critical for the uptake of cholesterol across the plasma membrane of the intestinal enterocyte. Is the direct molecular target of ezetimibe, a drug that inhibits cholesterol absorbtion. Lack of activity leads to multiple lipid transport defects. The protein may have a function in the transport of multiple lipids and their homeostasis, and may play a critical role in regulating lipid metabolism. -
Tissue specificity
Widely expressed. Expressed in liver. Also expressed in small intestine, pancreas, kidney, lung, pancreas, spleen, heart, gall bladder, brain, testis, stomach and muscle. -
Sequence similarities
Belongs to the patched family.
Contains 1 SSD (sterol-sensing) domain. -
Post-translational
modificationsHighly glycosylated. -
Cellular localization
Apical cell membrane. Cell membrane. Cytoplasmic vesicle membrane. Subfractionation of brush border membranes from proximal enterocytes suggests considerable association with the apical membrane fraction. Exists as a predominantly cell surface membrane expressed protein (By similarity). According to PubMed:15671032, localizes in a subcellular vesicular compartment rich in RAB5. - Information by UniProt
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Database links
- Entrez Gene: 29881 Human
- Omim: 608010 Human
- SwissProt: Q9UHC9 Human
- Unigene: 567486 Human
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Alternative names
- Niemann Pick C1 like protein 1 antibody
- Niemann Pick disease type C1 gene like 1 antibody
- Niemann-Pick C1-like protein 1 antibody
see all
Images
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Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (ab124801) at 1/1000 dilution (purified) + HeLa whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 149 kDa
Observed band size: 149 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (ab124801) at 1/10000 dilution (purified) + Human fetal liver tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 149 kDa
Observed band size: 149 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
All lanes : Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (ab124801) at 1/1000 dilution (unpurified)
Lane 1 : Human fetal liver lysates
Lane 2 : HeLa lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 149 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab124801 has been referenced in 8 publications.
- Hong J et al. The Effects of Anthocyanin-Rich Bilberry Extract on Transintestinal Cholesterol Excretion. Foods 10:N/A (2021). PubMed: 34829135
- Chen X et al. Interaction between Flavonoids and Carotenoids on Ameliorating Oxidative Stress and Cellular Uptake in Different Cells. Foods 10:N/A (2021). PubMed: 34945647
- Zang R et al. Cholesterol 25-hydroxylase suppresses SARS-CoV-2 replication by blocking membrane fusion. Proc Natl Acad Sci U S A 117:32105-32113 (2020). PubMed: 33239446
- Wang Y et al. Hepatic NPC1L1 overexpression attenuates alcoholic autophagy in mice. Mol Med Rep 20:3224-3232 (2019). PubMed: 31432115
- Yang F et al. Fatty acids modulate the expression levels of key proteins for cholesterol absorption in Caco-2 monolayer. Lipids Health Dis 17:32 (2018). PubMed: 29463265
- Yang F et al. Egg-Yolk Sphingomyelin and Phosphatidylcholine Attenuate Cholesterol Absorption in Caco-2 Cells. Lipids 53:217-233 (2018). PubMed: 29569242
- Hu YW et al. A lincRNA-DYNLRB2-2/GPR119/GLP-1R/ABCA1-dependent signal transduction pathway is essential for the regulation of cholesterol homeostasis. J Lipid Res 55:681-97 (2014). PubMed: 24493833
- Renner O et al. Role of the ABCG8 19H risk allele in cholesterol absorption and gallstone disease. BMC Gastroenterol 13:30 (2013). WB . PubMed: 23406058