Anti-Niemann Pick C1 Like 1/NPC1L1 antibody (ab121000)
Key features and details
- Goat polyclonal to Niemann Pick C1 Like 1/NPC1L1
- Suitable for: WB
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Niemann Pick C1 Like 1/NPC1L1 antibody
See all Niemann Pick C1 Like 1/NPC1L1 primary antibodies -
Description
Goat polyclonal to Niemann Pick C1 Like 1/NPC1L1This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
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Host species
Goat -
Species reactivity
Predicted to work with: Mouse, Rat, Rabbit, Human, Cynomolgus monkey, Chinese hamster -
Immunogen
Synthetic peptide corresponding to Mouse Niemann Pick C1 Like 1/NPC1L1 aa 50-150 (N terminal).
Database link: NP_997125.2 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 147 kDa.
A 1 hour primary incubation is recommended for this product. Preliminary experiments in Mouse and Rat Liver lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would welcome your feedback if you have tested this antibody in more suitable positive controls. |
Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 147 kDa. A 1 hour primary incubation is recommended for this product. Preliminary experiments in Mouse and Rat Liver lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would welcome your feedback if you have tested this antibody in more suitable positive controls. |
Target
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Function
Play a major role in cholesterol homeostasis. Is critical for the uptake of cholesterol across the plasma membrane of the intestinal enterocyte. Is the direct molecular target of ezetimibe, a drug that inhibits cholesterol absorbtion. Lack of activity leads to multiple lipid transport defects. The protein may have a function in the transport of multiple lipids and their homeostasis, and may play a critical role in regulating lipid metabolism. -
Tissue specificity
Widely expressed. Expressed in liver. Also expressed in small intestine, pancreas, kidney, lung, pancreas, spleen, heart, gall bladder, brain, testis, stomach and muscle. -
Sequence similarities
Belongs to the patched family.
Contains 1 SSD (sterol-sensing) domain. -
Post-translational
modificationsHighly glycosylated. -
Cellular localization
Apical cell membrane. Cell membrane. Cytoplasmic vesicle membrane. Subfractionation of brush border membranes from proximal enterocytes suggests considerable association with the apical membrane fraction. Exists as a predominantly cell surface membrane expressed protein (By similarity). According to PubMed:15671032, localizes in a subcellular vesicular compartment rich in RAB5. - Information by UniProt
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Database links
- Entrez Gene: 29881 Human
- Entrez Gene: 237636 Mouse
- Entrez Gene: 432367 Rat
- Omim: 608010 Human
- SwissProt: Q9UHC9 Human
- SwissProt: Q6T3U4 Mouse
- SwissProt: Q6T3U3 Rat
- Unigene: 567486 Human
see all -
Alternative names
- Niemann Pick C1 like protein 1 antibody
- Niemann Pick disease type C1 gene like 1 antibody
- Niemann-Pick C1-like protein 1 antibody
see all
Datasheets and documents
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Datasheet download
References (2)
ab121000 has been referenced in 2 publications.
- Huang WW et al. Cis- and Trans-Palmitoleic Acid Isomers Regulate Cholesterol Metabolism in Different Ways. Front Pharmacol 11:602115 (2020). PubMed: 33363473
- Zhong CY et al. Microbiota prevents cholesterol loss from the body by regulating host gene expression in mice. Sci Rep 5:10512 (2015). WB ; Mouse . PubMed: 26015368