Anti-Ninein antibody - Centrosome Marker (ab4447)

  - Ninein antibody - Centrosome Marker (ab4447)

IF using ab4447 to detect Ninein in MCF7 cells (human).

The left hand panels are DAPI stained nuclei. The right hand panels are the same nuclei stained for Ninein.

ab4447 was used at  1/1000.

Ninein shows nuclear and centrosome staining, which is expected from the literature.

Gordon Chan, University of Alberta

  Immunocytochemistry/ Immunofluorescence - Ninein antibody - Centrosome Marker (ab4447)

ICC/IF image of ab4447 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab4447, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iT^TM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

ab4447 shows the characteristic nuclear and centrosome staining for Ninein.