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Nitric Oxide Assay Kit (Colorimetric) (ab65328)

Q&A (26)Specific References (2)

Overview

  • Product nameNitric Oxide Assay Kit (Colorimetric)See all Nitric oxide kits ...
  • Tests
    200 x 1 assay
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay typeQuantitative
  • Sensitivity
    > 0.1 nmol/well
  • Range
    0.1 nmol/well - 10 nmol/well
  • Assay time
    1h 30m
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam's Nitric Oxide Assay Kit (Colorimetric) provides an accurate, convenient measure of total nitrate/nitrite in a simple two-step process. The first step converts nitrate to nitrite utilizing nitrate reductase. The second step uses Griess Reagents to convert nitrite to a deep purple azo compound. The amount of the azochromophore accurately reflects nitric oxide amount in samples.
    Visit our FAQs page for tips and troubleshooting.

     

    If you are interested in a fluorometric detection kit, please check Nitric Oxide Assay Kit (Fluorometric) (ab65327).

  • Notes

    Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. NO is rapidly oxidized to nitrite and nitrate which are used to quantitate NO production.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceNitric oxide (NO) is a key vertebrate biological messenger, playing an important role in neurotransmission, vascular regulation, immune responses and apoptosis. NO , also known as "endothelium-derived relaxing factor" or "EDRF", is synthesized from L-arginine, oxygen and NADPH by various NO synthases. Most of the NO in the cell is oxidized to nitrite (NO2-) and nitrate (NO3-), and therefore the concentrations of these anions are generally as a quantitative measure of NO production.

    • Applications

    Our Abpromise guarantee covers the use of ab65328 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Nitric Oxide Assay Kit (Colorimetric) images

    • Functional Studies - Nitric Oxide Colorimetric Assay Kit (ab65328)
      Functional Studies - Nitric Oxide Colorimetric Assay Kit (ab65328)
      Standard calibration Curve of nitrate (nitrite).

    Protocols

    References for Nitric Oxide Assay Kit (Colorimetric) (ab65328)

    This product has been referenced in:
    • Chavez-Valdez R  et al. Necrostatin-1 attenuates mitochondrial dysfunction in neurons and astrocytes following neonatal hypoxia-ischemia. Neuroscience 219:192-203 (2012). Read more (PubMed: 22579794) »
    • Hoetzenecker K  et al. Secretome of apoptotic peripheral blood cells (APOSEC) attenuates microvascular obstruction in a porcine closed chest reperfused acute myocardial infarction model: role of platelet aggregation and vasodilation. Basic Res Cardiol 107:292 (2012). Read more (PubMed: 22899170) »

    See all 2 Publications for this product

    Publishing research using ab65328 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab65328 Submit a Question for ab65328

    Displaying 1 - 10 of 26 results for Abreviews and Q&A

    10
    Votes: Highest First

    Inquiry: My assay buffer got spilled what can I use to replace it? Is PBS ok?



    I am happy to confirm that extra assay buffer can now be purchased using the following product number:

    ab171368

    http://www.abcam.com/index.html?datasheet=171368 (or use the following: http://www.abcam.com/index.html?datasheet...

    Read More

    I was wondering if you could let me know if the product above includes a step to remove proteins? Or if it needs one?


    This kit does not include a step to remove proteins. Depending on your sample type, it is however recommended to use 10kD spin columns (ab93349) to deproteinate your samples.

    For example, it is recommended for serum samples since there are ...

    Read More

    Inquiry: Hi, I am interested in quantifying the amount of NO in my cell lysates. I was wondering what methods of lysis are approved for this (65328) kit. I work in a BL3 setting, but do not have access to a plate reader within the BL3 itself, and therefor...

    Read More

    Thank you for contacting us.


    We have not tried boiling the end products to see if it affects the readings, but I would guess that it would. I would think that the safest way to use these samples and maintain the analysis efficiency would be ...

    Read More

    Product code: 65328
    Inquiry: I see that in several of the Scientific Support question-and-answers, the kit was tested in plasma. Is serum preferable to plasma for any reason? How long can the whole blood (venous, human) sit at room temperature before ...

    Read More

    Thank you for contacting us.

    The kits is absolutely fine to be used with both plasma and serum.

    30 minutes clotting time will be OK at room temperature for separation of serum.

    You can collect blood straightaway in EDTA containi...

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    What kind of lysis buffer should be used when preparing cell lysates for the kit?
    Is 1 hour really long enough to convert the nitrate to nitrite?
    For the standard preparations, does this really correspond to 0, 2, 4, 6, 7, and 10 nmol/well?

    You can use the assay buffer for lysis. The 1hr time period has been optimized for best results. However, you can increase the time slightly if you think that would help your assay.

    For the standard preparation, keep in mind that the final stand...

    Read More

    Here is the answer from our lab manager.
    1. For the standards, why are there two columns for nitrate and nitrite? Yes
    2. The nitrite curve looks wrong. Also, was the DMSO and H2O2 not used just as a vehicle control for the drug? Yes
    3. More...

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    Thank you very much for your reply.
    Could you please check as to why there are two columns each for the nitrate and nitrite standard curves? The nitrite standard curve should be without the NO reductase, and the nitrate standard curve should be with ...

    Read More

    Dear Technical,

    I do have some question. One of our researcher use the No Assay kit, which is colorimetric products.

    I do have some question

    1. He have bad result. Could you give me some tips for the data? The attached fil...

    Read More

    Thank you very much for contacting us with your enquiry.
    I've sent the protocol and data over to the lab scientists to review, and they do have some additional questions and comments about the data.

    "For the standards, why are there two co...

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    I would like to receive a replacement kit.

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this ha...

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    I haven't heard back from anyone in a couple of days. I was just checking to see what the progress was on the kit inquiry.

    Thank you for your patience with this reply. I will be helping you with this while Carolyn is out of the office.

    The lab does think that one of the kit components lost partial efficiency which is why it took a long time for color to develop. The...

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    appel- est-ce que les échantillons (surnagéants de culture)peuvent être congélées avant l'experience?

    Merci pour votre patience.

    Le laboratoire vient de me confirmer que le NO est stable et que les échantillons de surnagéant aussi peuvent donc être conservées en aliquots à-20°C ou -80°C.

    J'esp&...

    Read More
    

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"