Anti-Nitrotyrosine antibody [HM.11] (ab7048)
Key features and details
- Mouse monoclonal [HM.11] to Nitrotyrosine
- Suitable for: IHC-Fr, IHC-P
- Reacts with: Species independent
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
Anti-Nitrotyrosine antibody [HM.11]
See all Nitrotyrosine primary antibodies -
Description
Mouse monoclonal [HM.11] to Nitrotyrosine -
Host species
Mouse -
Specificity
This antibody reacts with nitrotyrosine, both with the free amino acid as well as with proteins containing nitrotyrosine. The antibody does not recognize other antigens with a nitrotyrosine-like structure such as tyrosine, 3-chlorotyrosine, phosphotyrosine and phenylalanine. -
Tested applications
Suitable for: IHC-Fr, IHC-Pmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to Nitro tyrosine (nitrated ) conjugated to Keyhole Limpet Haemocyanin (KLH).
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Positive control
- WB: mouse kidney lysate, mouse optic nerve, retina , spinal cord and brain lysates, rat aorta lysate(see Abreviews) IHC-P: human lung tissue (see Abreview)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: PBS, 0.1% BSA
WARNING!!: Do not add sodium azide or nitric containing perservative as it will damage the antibody. -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
HM.11 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7048 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
Acetone fixation 10 min -20 degree C; block endogenous peroxidase by 0.3 % H2O2 in PBS (or methanol for intracellular staining); block with 10% NGS or 5 % BSA for 30 min. |
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IHC-P | (3) |
1/200 - 1/400. 10% formalin fixation; 3% H2O2 to block endogenous peroxidases; Citrate buffer pH 6.0 for 1 min at 100°C as antigen retrieval treatment
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Notes |
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IHC-Fr
Use at an assay dependent concentration. Acetone fixation 10 min -20 degree C; block endogenous peroxidase by 0.3 % H2O2 in PBS (or methanol for intracellular staining); block with 10% NGS or 5 % BSA for 30 min. |
IHC-P
1/200 - 1/400. 10% formalin fixation; 3% H2O2 to block endogenous peroxidases; Citrate buffer pH 6.0 for 1 min at 100°C as antigen retrieval treatment |
Target
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Relevance
The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected, estimated semi-quantitatively and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.
Images
Datasheets and documents
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SDS download
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Datasheet download
References (70)
ab7048 has been referenced in 70 publications.
- Tryfonos A et al. Association between atherogenic risk-modulating proteins and endothelium-dependent flow-mediated dilation in coronary artery disease patients. Eur J Appl Physiol 123:367-380 (2023). PubMed: 36305972
- D'Haese S et al. Acute Exposure to Glycated Proteins Impaired in the Endothelium-Dependent Aortic Relaxation: A Matter of Oxidative Stress. Int J Mol Sci 23:N/A (2022). PubMed: 36499244
- Jang J et al. Interleukin-4 Aggravates LPS-Induced Striatal Neurodegeneration In Vivo via Oxidative Stress and Polarization of Microglia/Macrophages. Int J Mol Sci 23:N/A (2022). PubMed: 35008995
- Pereira PDS et al. Haptoglobin treatment contributes to regulating nitric oxide signal and reduces oxidative stress in the penis: A preventive treatment for priapism in sickle cell disease. Front Physiol 13:961534 (2022). PubMed: 36176769
- Liou GG et al. N-Acetyl Cysteine Overdose Inducing Hepatic Steatosis and Systemic Inflammation in Both Propacetamol-Induced Hepatotoxic and Normal Mice. Antioxidants (Basel) 10:N/A (2021). PubMed: 33809388