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Nitroreductase protein (Active) (ab59673)

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Overview

  • Product nameNitroreductase protein (Active)See all Nitroreductase proteins and peptides ...
  • Protein descriptionGene for (Escherichia coli) enzyme NTR is HIS tagged and expressed in a competent (Escherichia coli) cell line, monomer MW 24kDa.
  • Expression hostE. coli

    • Properties

  • Purity> 90 % by SDS-PAGE
  • FormLyophilised:Reconstitute the contents of the vial in 0.1ml water.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: PBS, Lactose
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab59673 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    Functional Studies
  • Application notesSDS-PAGE: Use at an assay dependent dilution.

    Enzyme Activity: Unit Definition: One unit will reduce one µmole of Cytochrome C per minute in the presence of Menadione and NADH at pH 7.4 at 37 °C. Minimum activity is 100units/mg protein.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Dihydropteridine reductasedprAFMN dependent nitroreductase
        nfnBnfsIntrOxygen insensitive NAD(P)H nitroreductase
      see all
  • RelevanceNitroreductase shows the ability to reduce quinines. It is an enzyme for activating prodrugs in antibody directed enzyme prodrug therapy. It is also capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5-(aziridin-1-yl)-2,4-dinitrobenzamide). The reduction of CB1954 results in the generation of cytotoxic species.

    • References for Nitroreductase protein (Active) (ab59673)

    ab59673 has not yet been referenced specifically in any publications.

    Publishing research using ab59673 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

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    Displaying 1 - 6 of 6 results for Abreviews and Q&A

    10
    Votes: Highest First

    Thank you for sending me the protocol for the nitroreductase assay. I noticed that the assay is carried out in 1 ml quartz cuvettes. I was wondering if it is possible to adapt the protocol so that the assay can be done in 96 well microtitre plates. Thanks...

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    Thank you for contacting us.

    Theoretically, the assay can be adapted to 96 cell plate as well. We unfortunately do not have protocol other than what we sent; you may need to optimize the protocol for better results.

    I hope this informati...

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    Inquiry: Hi, I am working with a nitroreductase and I need to assay its activity. I want to use the active nitroreductase protein sold through abcam as a positive control. Can you please email me the nitroreductase assay protocol that you use to measure t...

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    Thank you for contacting us.

    Please find attached the protcol.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Ear...

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    Thank you for your reply! It is actually same procedure for enzyme assay as that of Aldrich-Sigma. You have tested the enzymatic activity and make sure it is active. Could you send the UV spectra for enzyme assay? I want to know about more information bef...

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    Thank you for your inquiry. I heard back from the supplying lab and unfortunately they do not have UV spectra for this product. The protein concentration and enzymatic activity are the only parameters tested. The vial contains 1 mg of protein, 20 mg lacto...

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    Thank you for your reply! I want to know about more information before we purchase the enzyme on next monday. 1mg enzyme in the vial should be dissolved/reconstituted with 0.1 mL H20 (if too less? Maybe suspension?), then aliquots and stored at -20degree...

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    Thank you for your reply. I have requested the UV spectra from the testing laboratory. The enzyme can be prepared in the following buffer: 10mg of BSA in 100ml of PBS pH 7.4. Further dilution information is found in the assay protocol. I have included thi...

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    Thank you very much! Do you test the reduction of anti-tumor agent CB1954(5-(aziridin-1-yl)-2,4dinitrobenzamide)using enzyme ab59673,which you mentioned in your description for the relevance of nitroreductase? Because we also want to reduce some aromatic ...

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    Thank you for your reply. We have not tested the reduction of CB1954 using ab59673 nor do we sell CB1954. That note from the relevance section is taken from the Swiss Prot entry for the protein. http://www.uniprot.org/uniprot/P38489 We only have tested th...

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    Enzyme Commission number (EC番号）を教えてください。

    I am sorry but I now realise I gave you incorrect information in my previous email; this is an E. Coli enzyme not human origin. The gene for the E. Coli enzyme NTR is HIS tagged and expressed in a competent E. Coli cell line. There is no EC number a...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"