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Product name
Nitrotyrosine ELISA Kit
Assay type
Competitive
Product overview
This nitrotyrosine (3-nitrotyrosine, 3NT) competitive ELISA is used to determine the level of nitrotyrosine modified proteins in a sample. The provided microplates have been evenly coated with a nitrotyrosine containing antigen. The competitive ELISA is performed by adding the test sample mixed with the provided HRP conjugated anti-3NT antibody. If no 3NT modified proteins are present in the sample, all of the anti-3NT detector antibody is available to bind to the immobilized 3NT containing protein coating the wells, generating a maximum (100%) binding signal. In contrast, if soluble 3NT modified protein is present in the sample, it will compete for binding by the anti-3NT detector antibody. The degree of competition is proportional to the concentration of soluble 3NT modified proteins in the sample. Therefore the signal in each well has an inverse relationship to the amount of 3NT in each sample. When performing each assay a standard curve is generated from the provided standard to allow the accurate quantitation of the 3NT content in the test samples.
The assay is followed by monitoring the HRP dependant color change in each well at 600 nm. Alternatively, the assay can be terminated, at a user-defined time, by the addition of 1N HCl (not supplied) and the assay performed as an end-point measurement at 450 nm
Tested applications
Competitive ELISAmore details
Properties
Storage instructions
Store at +4°C.
| Components | Identifier | 2 x 96 tests |
|---|---|---|
| 3NT-BSA Standard | P/N 8209517 | 1 vial |
| Blocking Solution (10X) | P/N 8209203 | 1 x 10ml |
| Development Solution (1X) | P/N 8209714 | 1 x 25ml |
| Extraction Buffer | P/N 8201093 | 1 x 15ml |
| HRP conjugated 3NT Detector Antibody (1000X) | P/N 8209516 | 1 x 0.04ml |
| Nitrotyrosine coated 96-well microplate (12 strips) | P/N 8209518 | 2 units |
| Plate Seals | 2 units | |
| Wash Buffer (20X) | P/N 8209204 | 1 x 25ml |
Research areas
Cell Biology >> Other Antibodies >> Oxidative Stress
Signal Transduction >> Metabolism >> Amino Acids
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Competitive ELISA - Nitrotyrosine ELISA Kit (ab113848)
(enlarge)
Alternative names
- ms792
Applications
Show applications key
Our Abpromise guarantee covers the use of ab113848 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
cELISA
Nitrotyrosine ELISA Kit images:
Competitive ELISA - Nitrotyrosine ELISA Kit (ab113848)
Example standard curve using 3NT standard in molar concentration.
Protocols
References for Nitrotyrosine ELISA Kit (ab113848)
ab113848 has not yet been referenced specifically in any publications.
Publishing research using ab113848? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"