Anti-Nkx2.2 antibody (ab86024)

Overview

• Product nameAnti-Nkx2.2 antibodySee all Nkx2.2 primary antibodies ...
• Description
  Rabbit polyclonal to Nkx2.2
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  3 synthetic peptides (Human) conjugated to KLH

• Positive controlRecombinant protein produced in an induced culture of E.coli.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
  Constituents: Whole serum
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Developmental Biology
  • Organogenesis
  • Gut development
  • Pancreas development

  • Stem Cells
  • Lineage Markers
  • Ectoderm

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • Developmental Families
  • Other

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Transcription Factors

  • Neuroscience
  • Neurology process
  • Growth and Development
  • Axonal Guidance Proteins

  • Neuroscience
  • Neurology process
  • Neurogenesis

Applications

Target

• FunctionMay be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance.
• Sequence similaritiesBelongs to the NK-2 homeobox family.
  Contains 1 homeobox DNA-binding domain.
• DomainThe homeodomain is essential for interaction with OLIG2.
• Cellular localizationNucleus.

Target information above from: UniProt accession O95096 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4821 Human
  • Entrez Gene: 18088 Mouse
  • Omim: 604612 Human
  • SwissProt: O95096 Human
  • SwissProt: P42586 Mouse
  • Unigene: 516922 Human
  • Unigene: 330639 Mouse

• Alternative names
  Homeobox protein NK 2 homolog B antibodyHomeobox protein NK-2 homolog B antibodyHomeobox protein Nkx 2.2 antibody

  Homeobox protein Nkx-2.2 antibodyNK 2 homolog B antibodyNK2 homeobox 2 antibodyNK2 transcription factor like protein B antibodyNK2 transcription factor related locus 2 antibodyNKX2 2 antibodyNKX2-2 antibodyNKX22 antibodyNKX22_HUMAN antibodyNkx2b antibodytinman antibody

  see all

Anti-Nkx2.2 antibody images

• 

  Western blot - Nkx2.2 antibody (ab86024)
  All lanes : Anti-Nkx2.2 antibody (ab86024) at 1/1000 dilution
  
  Lane 1 : An uninduced (negative control) culture of E.coli
  Lane 2 : An induced culture of E.coli
  
  Secondary
  Anti-Rabbit IgG HRP at 1/1000 dilution
  
  Predicted band size : 30 kDa
  Observed band size : 53 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 130 kDa,35 kDa,37 kDa,42 kDa. We are unsure as to the identity of these extra bands.