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Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Overview
Product name
Anti-Nkx3.1 antibody
See all Nkx3.1 products (8) ...
Description
Rabbit polyclonal to Nkx3.1
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to a region within C terminal residues 180 - 229 (KRKQLSSELG DLEKHSSLPA LKEEAFSRAS LVSVYNSYPY YPYLYCVGSW) of Human Nkx3.1 (NP_006158)
KRKQLSSELG DLEKHSSLPA LKEEAFSRAS LVSVYNSYPY YPYLYCVGSW
Positive control
293T cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
-
Western blot - Nkx3.1 antibody (ab83973)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab83973 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 26 kDa.
Target
Function
Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Play an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Act as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells.
Tissue specificity
Highly expressed in the prostate and, at a lower level, in the testis.
Sequence similarities
Belongs to the NK-3 homeobox family.
Contains 1 homeobox DNA-binding domain.
Post-translational
modifications
Ubiquitinated by TOPORS; monoubiquitinated at several residues and also polyubiquitinated on single residues.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ99801
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BAPX 2 antibody
- BAPX2 antibody
- Homeobox protein NK-3 homolog A antibody
see all
Database links
- Entrez Gene: 4824 Human
- GenBank: NM_006167 Human
- Omim: 602041 Human
- SwissProt: Q99801 Human
- Unigene: 55999 Human
Anti-Nkx3.1 antibody images:
Western blot - Nkx3.1 antibody (ab83973)
Anti-Nkx3.1 antibody (ab83973) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 26 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)
12% gel
References for Anti-Nkx3.1 antibody (ab83973)
ab83973 has not yet been referenced specifically in any publications.
Publishing research using ab83973? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"