Anti-Nmnat1 antibody [1F7] (ab118270)

Overview

• Product nameAnti-Nmnat1 antibody [1F7]See all Nmnat1 primary antibodies ...
• Description
  Mouse monoclonal [1F7] to Nmnat1
• Tested applicationsWB, ICC/IF more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant full length protein, corresponding to amino acids 1-279 of Human Nmnat1, produced in HEK293T cells (NP_073624).

• Positive controlNmnat1 transfected HEK293T cell lysate and COS7 cells transiently transfected with Nmnat1

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferpH: 7.30
  Preservative: 0.02% Sodium azide
  Constituents: 48% PBS, 1% BSA, 50% Glycerol
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesPurified from Mouse ascites fluids by affinity chromatography.
• Clonality Monoclonal
• Clone number1F7
• IsotypeIgG1
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Energy Metabolism

  • Signal Transduction
  • Metabolism
  • Energy Metabolism

  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • ADP-ribosylation

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Other

Applications

Target

• FunctionCatalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity, prefers NAD(+) and NAAD as substrates and degrades NADH, nicotinic acid adenine dinucleotide phosphate (NHD) and nicotinamide guanine dinucleotide (NGD) less effectively. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NAADP(+). Protects against axonal degeneration following mechanical or toxic insults.
• Tissue specificityWidely expressed with highest levels in skeletal muscle, heart and kidney. Also expressed in the liver pancreas and placenta. Widely expressed throughout the brain.
• PathwayCofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1.
• Involvement in diseaseDefects in NMNAT1 are the cause of Leber congenital amaurosis 9 (LCA9) [MIM:608553]. A severe dystrophy of the retina, typically becoming evident in the first years of life. Visual function is usually poor and often accompanied by nystagmus, sluggish or near-absent pupillary responses, photophobia, high hyperopia and keratoconus.
• Sequence similaritiesBelongs to the eukaryotic NMN adenylyltransferase family.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q9HAN9 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 64802 Human
  • Omim: 608700 Human
  • SwissProt: Q9HAN9 Human
  • Unigene: 633762 Human

• Alternative names
  NaMN adenylyltransferase 1 antibodyNicotinamide mononucleotide adenylyltransferase 1 antibodynicotinamide nucleotide adenylyltransferase 1 antibody

  nicotinate nucleotide adenylyltransferase 1 antibodyNicotinate-nucleotide adenylyltransferase 1 antibodyNMN adenylyltransferase 1 antibodyNMNA1_HUMAN antibodyNmnat 1 antibodyNmnat1 antibodyOTTHUMP00000001731 antibodyOTTHUMP00000001732 antibodyOTTHUMP00000035892 antibodyPNAT 1 antibodyPNAT1 antibodypyridine nucleotide adenylyltransferase 1 antibody

  see all

Anti-Nmnat1 antibody [1F7] images

• 

  Western blot - Anti-Nmnat1 antibody [1F7] (ab118270)
  All lanes : Anti-Nmnat1 antibody [1F7] (ab118270) at 1/2000 dilution
  
  Lane 1 : Non transfected cell lysate
  Lane 2 : Nmnat1 transfected HEK293T cell lysate
  
  Lysates/proteins at 5 µg per lane.
  
  
  Predicted band size : 31 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - Anti-Nmnat1 antibody [1F7] (ab118270)
  ab118270, at 1/100, staining Nmnat1 in COS7 cells transiently transfected with Nmnat1, by immunofluorescence.