Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab16054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
IHC-P 1/175. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 26, 35 kDa (predicted molecular weight: 26 kDa).Can be blocked with Human Noggin peptide (ab16380).
  • Application notesIs unsuitable for IHC-Fr.
  • Target

    • FunctionEssential for cartilage morphogenesis and joint formation. Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite.
    • Involvement in diseaseDefects in NOG are a cause of symphalangism proximal syndrome (SYM1) [MIM:185800]. SYM1 is characterized by the hereditary absence of the proximal interphalangeal (PIP) joints (Cushing symphalangism). Severity of PIP joint involvement diminishes towards the radial side. Distal interphalangeal joints are less frequently involved and metacarpophalangeal joints are rarely affected whereas carpal bone malformation and fusion are common. In the lower extremities, tarsal bone coalition is common. Conducive hearing loss is seen and is due to fusion of the stapes to the petrous part of the temporal bone.
      Defects in NOG are the cause of multiple synostoses syndrome type 1 (SYNS1) [MIM:186500]; also known as synostoses, multiple, with brachydactyly/symphalangism-brachydactyly syndrome. SYNS1 is characterized by tubular-shaped (hemicylindrical) nose with lack of alar flare, otosclerotic deafness, and multiple progressive joint fusions commencing in the hand. The joint fusions are progressive, commencing in the fifth proximal interphalangeal joint in early childhood (or at birth in some individuals) and progressing in an ulnar-to-radial and proximal-to-distal direction. With increasing age, ankylosis of other joints, including the cervical vertebrae, hips, and humeroradial joints, develop.
      Defects in NOG are the cause of tarsal-carpal coalition syndrome (TCC) [MIM:186570]. TCC is an autosomal dominant disorder characterized by fusion of the carpals, tarsals and phalanges, short first metacarpals causing brachydactyly, and humeroradial fusion. TCC is allelic to SYM1, and different mutations in NOG can result in either TCC or SYM1 in different families.
      Defects in NOG are a cause of stapes ankylosis with broad thumb and toes (SABTS) [MIM:184460]; also known as Teunissen-Cremers syndrome. SABTS is a congenital autosomal dominant disorder that includes hyperopia, a hemicylindrical nose, broad thumbs, great toes, and other minor skeletal anomalies but lacked carpal and tarsal fusion and symphalangism.
      Defects in NOG are the cause of brachydactyly type B2 (BDB2) [MIM:611377]. BDB2 is a subtype of brachydactyly characterized by hypoplasia/aplasia of distal phalanges in combination with distal symphalangism, fusion of carpal/tarsal bones, and partial cutaneous syndactyly.
    • Sequence similaritiesBelongs to the noggin family.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • NOG antibody
      • NOGG_HUMAN antibody
      • Noggin antibody
      • SYM 1 antibody
      • SYM1 antibody
      • Symphalangism 1 (proximal) antibody
      • Synostoses (multiple) syndrome 1 antibody
      • SYNS 1 antibody
      • SYNS1 antibody
      see all

    Anti-Noggin antibody images

    • All lanes : Anti-Noggin antibody (ab16054) at 1 µg/ml

      Lane 1 : Noggin Mouse Recombinant Protein
      Lane 2 : Noggin Mouse Recombinant Protein with Human Noggin peptide (ab16380) at 1 µg/ml

      Lysates/proteins at 0.01 µg per lane.


      Predicted band size : 26 kDa
      Observed band size : 26 kDa
    • Image courtesy of Human Protein Atlas
      ab16054 stainning Noggin in paraffin-embedded human liver tissue, showing a cytoplasmic and/or membranous distribution in both hepatocytes and bile duct cells. Paraffin embedded tissue was incubated with ab16054 (1/175 dilution) for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
      ab16054 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
    • ICC/IF image of Noggin stained primary myoblast cells. The cells were 4% PFA fixed (10 min) and then incubated in 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tritonx100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16054, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488donkey anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h.. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

      See Abreview

    • All lanes : Anti-Noggin antibody (ab16054) at 1 µg/ml

      Lane 1 : Noggin Human Recombinant Protein
      Lane 2 : Noggin Mouse Recombinant Protein

      Lysates/proteins at 0.1 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 26 kDa
      Observed band size : 26,35 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 minute
    • Anti-Noggin antibody (ab16054) at 1 µg/ml + Active human Noggin full length protein (ab73756) at 1 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 4 minutes

    References for Anti-Noggin antibody (ab16054)

    This product has been referenced in:
    • Sanges D  et al. Wnt/ß-catenin signaling triggers neuron reprogramming and regeneration in the mouse retina. Cell Rep 4:271-86 (2013). Read more (PubMed: 23850287) »
    • Cao H  et al. The Pitx2:miR-200c/141:noggin pathway regulates Bmp signaling and ameloblast differentiation. Development 140:3348-59 (2013). IP . Read more (PubMed: 23863486) »

    See all 7 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA, pH8.6, 100C, 20 min
    Sample Human Tissue sections (small intestine)
    Specification small intestine
    Blocking step hydrogen peroxide as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jul 17 2014

    Thank you for your call today and for letting us know about the problems with these antibodies. As we discussed, I am sending free of charge replacement vials of a new lot of ab16054 and ab93855, which should arrive on the order *** Ple...

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    Application Western blot
    Sample Rat Tissue lysate - whole (liver, muscle, brain)
    Loading amount 20 µg
    Specification liver, muscle, brain
    Gel Running Conditions Reduced Denaturing (15% SDS-Polyacrylamide)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Sep 08 2010

    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Tissue sections (Myoblast culture)
    Specification Myoblast culture
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.3% TritonX
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Aug 26 2010

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Adult skin)
    Specification Adult skin
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric buffer, pH6
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 25 2010

    Application Western blot
    Sample Mouse Cell lysate - whole cell (Brain)
    Loading amount 10 µg
    Specification Brain
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 21 2009

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Kidney)
    Specification Kidney
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
    Blocking step Serum Free Protein Block as blocking agent for 20 minute(s) · Concentration: 100%
    Username

    Abcam user community

    Verified customer

    Submitted May 20 2009

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (fetal lung)
    Specification fetal lung
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6
    Permeabilization No
    Username

    Dr. mark southwood

    Verified customer

    Submitted Aug 27 2008

    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (e14.5 brain)
    Specification e14.5 brain
    Fixative Paraformaldehyde
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Dr. Dean Griffiths

    Verified customer

    Submitted Jan 04 2007

    Thank you for your enquiry. I have done a sequence alignment search for you on the immunogen for this antibody. Unfortunately, it only has 66% sequence alignment to both zebrafish nog 1 and 3. We usually recommend that sequence alignment is over 85% to...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"