Overview
- Product nameAnti-Noggin antibodySee all Noggin primary antibodies ...
- DescriptionRabbit polyclonal to Noggin
- Tested applicationsICC/IF, IHC-P, WB more details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Horse, Chicken, Xenopus laevis - Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Noggin.
(Peptide available as ab16380.)
- Positive controlThis antibody gave a positive signal in both Human and Mouse Noggin Recombinant protein.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab16054 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: Use at an assay dependent dilution. |
| IHC-P | IHC-P: 1/175. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
| WB | WB: Use a concentration of 1 µg/ml. Detects a band of approximately 26, 35 kDa (predicted molecular weight: 26 kDa).Can be blocked with Noggin peptide (ab16380). |
Target
- FunctionEssential for cartilage morphogenesis and joint formation. Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite.
- Involvement in diseaseDefects in NOG are a cause of symphalangism proximal syndrome (SYM1) [MIM:185800]. SYM1 is characterized by the hereditary absence of the proximal interphalangeal (PIP) joints (Cushing symphalangism). Severity of PIP joint involvement diminishes towards the radial side. Distal interphalangeal joints are less frequently involved and metacarpophalangeal joints are rarely affected whereas carpal bone malformation and fusion are common. In the lower extremities, tarsal bone coalition is common. Conducive hearing loss is seen and is due to fusion of the stapes to the petrous part of the temporal bone.
Defects in NOG are the cause of multiple synostoses syndrome type 1 (SYNS1) [MIM:186500]; also known as synostoses, multiple, with brachydactyly/symphalangism-brachydactyly syndrome. SYNS1 is characterized by tubular-shaped (hemicylindrical) nose with lack of alar flare, otosclerotic deafness, and multiple progressive joint fusions commencing in the hand. The joint fusions are progressive, commencing in the fifth proximal interphalangeal joint in early childhood (or at birth in some individuals) and progressing in an ulnar-to-radial and proximal-to-distal direction. With increasing age, ankylosis of other joints, including the cervical vertebrae, hips, and humeroradial joints, develop.
Defects in NOG are the cause of tarsal-carpal coalition syndrome (TCC) [MIM:186570]. TCC is an autosomal dominant disorder characterized by fusion of the carpals, tarsals and phalanges, short first metacarpals causing brachydactyly, and humeroradial fusion. TCC is allelic to SYM1, and different mutations in NOG can result in either TCC or SYM1 in different families.
Defects in NOG are a cause of stapes ankylosis with broad thumb and toes (SABTS) [MIM:184460]; also known as Teunissen-Cremers syndrome. SABTS is a congenital autosomal dominant disorder that includes hyperopia, a hemicylindrical nose, broad thumbs, great toes, and other minor skeletal anomalies but lacked carpal and tarsal fusion and symphalangism.
Defects in NOG are the cause of brachydactyly type B2 (BDB2) [MIM:611377]. BDB2 is a subtype of brachydactyly characterized by hypoplasia/aplasia of distal phalanges in combination with distal symphalangism, fusion of carpal/tarsal bones, and partial cutaneous syndactyly. - Sequence similaritiesBelongs to the noggin family.
- Cellular localizationSecreted.
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Database links
- Entrez Gene: 373912 Chicken
- Entrez Gene: 9241 Human
- Entrez Gene: 18121 Mouse
- Entrez Gene: 373646 Xenopus laevis
- Omim: 602991 Human
- SwissProt: O93525 Chicken
- SwissProt: Q13253 Human
- SwissProt: P97466 Mouse
- SwissProt: P49011 Xenopus laevis
- Unigene: 248201 Human
- Unigene: 135266 Mouse
- Unigene: 834 Xenopus laevis
see all
Target information above from: UniProt accession
Q13253
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- NOG antibodyNOGG_HUMAN antibodyNoggin antibody
- SYM 1 antibodySYM1 antibodySymphalangism 1 (proximal) antibodySynostoses (multiple) syndrome 1 antibodySYNS 1 antibodySYNS1 antibody
see all
Anti-Noggin antibody images
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Western blot - Noggin antibody (ab16054)All lanes : Anti-Noggin antibody (ab16054) at 1 µg/ml
Lane 1 : Noggin Mouse Recombinant Protein
Lane 2 : Noggin Mouse Recombinant Protein withNoggin peptide (ab16380) at 1 µg/ml
Lysates/proteins at 0.01 µg per lane.
Predicted band size : 26 kDa
Observed band size : 26 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Noggin antibody (ab16054)Image courtesy of Human Protein Atlas
ab16054 stainning Noggin in paraffin-embedded human liver tissue, showing a cytoplasmic and/or membranous distribution in both hepatocytes and bile duct cells. Paraffin embedded tissue was incubated with ab16054 (1/175 dilution) for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.ab16054 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org -
Immunocytochemistry/ Immunofluorescence - Noggin antibody (ab16054)Ruma Raha-Chowdhury, University Of Cambridge, United KingdomICC/IF image of Noggin stained primary myoblast cells. The cells were 4% PFA fixed (10 min) and then incubated in 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tritonx100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16054, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488donkey anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h.. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Western blot - Noggin antibody (ab16054)All lanes : Anti-Noggin antibody (ab16054) at 1 µg/ml
Lane 1 : Noggin Human Recombinant Protein
Lane 2 : Noggin Mouse Recombinant Protein
Lysates/proteins at 0.1 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 26 kDa
Observed band size : 26,35 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute -
Western blot - Anti-Noggin antibody (ab16054)Anti-Noggin antibody (ab16054) at 1 µg/ml +Noggin protein (Active) (ab73756) at 1 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 4 minutes
References for Anti-Noggin antibody (ab16054)
This product has been referenced in:
- Rong L et al. GATA-6 promotes cell survival by up-regulating BMP-2 expression during embryonic stem cell differentiation. Mol Biol Cell 23:3754-63 (2012). Read more (PubMed: 22855527) »
- Glister C et al. Granulosal and thecal expression of bone morphogenetic protein- and activin-binding protein mRNA transcripts during bovine follicle development and factors modulating their expression in vitro. Reproduction 142:581-91 (2011). Read more (PubMed: 21821720) »
