Non-Homologous End Joining Panel (H2A.X, gamma H2A.X pS139, DNA Ligase IV, Ku70, Ku80) (ab103983)

Overview

  • Product nameNon-Homologous End Joining Panel (H2A.X, gamma H2A.X pS139, DNA Ligase IV, Ku70, Ku80)
  • Product overviewab103983 is a Non Homologous End Joining Panel designed for the detection and characterization of key proteins involved in DNA double strand break (DSB) repair by non-homologous end joining (NHEJ). Histone H2A.X is phosphorylated on S139, termed gamma H2A.X, at sites of DNA damage. DNA DSB repair by NHEJ requires Ku70/Ku80 heterodimer binding to the DSB, as well as DNA Ligase IV for ligation of DNA ends.
  • Components

      Non-Homologous End Joining Panel (H2A.X, gamma H2A.X pS139, DNA Ligase IV, Ku70, Ku80) images

      • Lanes 1 & 3 : Anti-Histone H2A.X antibody (ab10475) at 1/500 dilution
        Lane 2 : Anti-Histone H2A.X antibody (ab10475) at 1/1000 dilution

        Lane 1 : Calf thymus histone lysate
        Lane 2 : Calf thymus histone lysate
        Lane 3 : Calf thymus histone lysate with Human Histone H2A.X (unmodified ) peptide (ab15020) at 1 µg

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

        Performed under reducing conditions.

        Exposure time : 30 seconds
      • All lanes : Anti-gamma H2A.X (phospho S139) antibody - ChIP Grade (ab2893) at 1/500 dilution

        Lane 1 : Control HeLa cell histone preparation
        Lane 2 : Colcemid treated HeLa histone preparation
        Lane 3 : Control HeLa cell histone preparation with Human gamma H2A.X (phospho S139) peptide (ab15645) at 1 µg
        Lane 4 : Colcemid treated HeLa histone preparation with Human gamma H2A.X (phospho S139) peptide (ab15645) at 1 µg
        Lane 5 : Control HeLa cell histone preparation with Human gamma H2A.X (unmodified ) peptide (ab15646) at 1 µg
        Lane 6 : Colcemid treated HeLa histone preparation with Human gamma H2A.X (unmodified ) peptide (ab15646) at 1 µg

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
      • All lanes : Anti-DNA Ligase IV antibody (ab80514) at 1 µg/ml

        Lane 1 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)
        Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
        developed using the ECL technique

        Performed under reducing conditions.

        Exposure time : 20 minutes
      • All lanes : Anti-Ku70 antibody (ab83501) at 1 µg/ml

        Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
        Lane 2 : Irradiated Hela (Control for ATM phospho S1981) Whole Cell Lysate
        Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
        Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
        Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
        Lane 6 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
        developed using the ECL technique

        Performed under reducing conditions.

        Exposure time : 1 minute
      • All lanes : Anti-Ku80 antibody (ab87860) at 1 µg/ml

        Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
        Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
        Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
        developed using the ECL technique

        Performed under reducing conditions.

        Exposure time : 3 minutes

      References for Non-Homologous End Joining Panel (H2A.X, gamma H2A.X pS139, DNA Ligase IV, Ku70, Ku80) (ab103983)

      This product has been referenced in:
      • Stimpson KM  et al. Telomere disruption results in non-random formation of de novo dicentric chromosomes involving acrocentric human chromosomes. PLoS Genet 6: (2010). Read more (PubMed: 20711355) »
      • Zhu H  et al. Involvement of Caveolin-1 in repair of DNA damage through both homologous recombination and non-homologous end joining. PLoS One 5:e12055 (2010). Read more (PubMed: 20700465) »

      See all 2 Publications for this product

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"