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Anti-Non Neuronal Enolase antibody
See all Non Neuronal Enolase products (8) ...
Rabbit polyclonal to Non Neuronal Enolase
NNE 100% NSE <0.1% Determined in a competitive ELISA with an antibody dilution of 1/500.
ELISA, IHC-P, WBmore details
Reacts with
Human
Full length Non Neuronal Enolase (Human)
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.2
Concentration information loading...
IgG fraction
Ammonium Sulphate fractionation
Polyclonal
IgG
Our Abpromise guarantee covers the use of ab35075 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/50 - 1/250. In a simple ELISA, with 1 µg of antigen coated per well, the 50% OD was observed at an antibody dilution of 1/120. Under the same conditions, the end point was observed at 1/10000.
IHC-P: 1/25 - 1/100.
WB: Use at an assay dependent dilution. Predicted molecular weight: 48 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
Belongs to the enolase family.
During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
ISGylated.
Nucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
Target information above from: UniProt accessionP06733
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
This product has been referenced in:
See all 2 publications for this product
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