Non Neuronal Enolase antibody (ab35075)

Product Name 

Non Neuronal Enolase antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Non Neuronal Enolase

Immunogen 

Full length Non Neuronal Enolase (Human)

Reacts with 
(species key)

Hu

Specificity 

NNE 100% NSE <0.1%
Determined in a competitive ELISA with an antibody dilution of 1/500.

Tested applications 
(see key)

ELISA, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ELISA: 1/50 - 1/250. In a simple ELISA, with 1 µg of antigen coated per well, the 50% OD was observed at an antibody dilution of 1/120. Under the same conditions, the end point was observed at 1/10000.
IHC-P: 1/25 - 1/100.
WB: Use at an assay dependent dilution. Predicted molecular weight: 48 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasm. Cell membrane. Isoform MBP-1: Nucleus. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.

Research areas 

Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Signal Transduction >> Metabolism >> Energy Metabolism

Relevance 

Non Neuronal Enolase (ENO1) is an isoform of mammalian enolase and is composed of 2 alpha subunits. The gene for ENO1 encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter.

Non Neuronal Enolase can be as a diagnostic marker for many tumors; as a marker for endometriosis and in the heterodimeric form, alpha/gamma, as a marker for hypoxic brain injury after cardiac arrest. It has been identified as an autoantigen in Hashimoto encephalopathy (associated with Hashimoto thyroiditis).

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Ammonium Sulphate fractionation

Form 

Liquid

Concentration 

5.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

References for Non Neuronal Enolase antibody (ab35075)

  This product has been used in:

Cohen AA et al. Dynamic proteomics of individual cancer cells in response to a drug. Science 322:1511-6 (2008). WB; Human. PubMed: 19023046

If you publish research using ab35075 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding Non Neuronal Enolase antibody

Customer FAQs regarding Non Neuronal Enolase antibody

Protocols for Non Neuronal Enolase antibody

Price and availability of products related to Non Neuronal Enolase antibody

Non Neuronal Enolase antibody - related products: