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Products:Signal Transduction >> Metabolism >> Energy Metabolism
Overview
Product name
Anti-Non Neuronal Enolase antibody
See all Non Neuronal Enolase products (8) ...
Description
Rabbit polyclonal to Non Neuronal Enolase
Specificity
This antibody is specific for Non Neuronal Enolase.
Tested applications
IHC-P, WB, IHC-Fr, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Pig, Zebrafish
Immunogen
A synthetic peptide corresponding to a region of Human Non Neuronal Enolase, derived from within the following sequence (aa 246-295): VAASEFFRSG KYDLDFKSPD DPSRYISPDQ LADLYKSFIK DYPVVSIEDP.
Positive control
Fetal Intestine lysate and human kidney tissue
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Co-factors
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Signal Transduction >> Metabolism >> Energy Metabolism
Applications
Show applications key
Our Abpromise guarantee covers the use of ab49343 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 4 - 8 µg/ml.
WB: Use a concentration of 0.03 µg/mlDetects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
IHC-Fr: 1/250
ICC/IF: Use a concentration of 1 µg/ml
Target
Function
Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
Tissue specificity
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
Sequence similarities
Belongs to the enolase family.
Developmental stage
During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
Post-translational
modifications
ISGylated.
Cellular localization
Nucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
Target information above from: UniProt accessionP06733
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 2-phospho-D-glycerate hydro-lyase antibody
- Alpha-enolase antibody
- bh3 interacting domain death agonist antibody
see all
Database links
- Entrez Gene: 2023 Human
- Entrez Gene: 100045967 Mouse
- Entrez Gene: 100503183 Mouse
- Entrez Gene: 13806 Mouse
- Entrez Gene: 433182 Mouse
- Entrez Gene: 406872 Pig
- Entrez Gene: 24333 Rat
- Entrez Gene: 334116 Zebrafish
see all
Anti-Non Neuronal Enolase antibody images:
Western blot - Non Neuronal Enolase antibody (ab49343)
Anti-Non Neuronal Enolase antibody (ab49343) at 0.03 µg/ml + Fetal intestine lysate 10µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 47 kDa
Observed band size : 47 kDa
Immunohistochemistry (Paraffin-embedded sections) - Non Neuronal Enolase antibody (ab49343)
ab49343 at 4µg/ml staining Non Neuronal Enolase in epithelial cells of collecting tubules (arrows) of human kidney tissue.
Immunohistochemistry (Frozen sections) - Non Neuronal Enolase antibody (ab49343)
ab49343 staining mouse embryonic brain slice tissue sections by IHC-Fr. Tissue sections were fixed with paraformaldehyde and permeabilized with 0.1% Triton-X in 0.1M PBS. The sample was blocked with 5% serum for 2 hours at 4°C prior to incubation with the primary antibody, diluted 1/20 in 0.1M PBS for 24 hours at 4°C. An Alexa Fluor® 546 conjugated goat anti-rabbit IgG antibody was used as the secondary.
This image is courtesy of an anonymous Abreview
Immunocytochemistry/ Immunofluorescence-Non Neuronal Enolase antibody(ab49343)
ICC/IF image of ab49343 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49343, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Non Neuronal Enolase antibody (ab49343)
This product has been referenced in:
- Deng MYet al. Frontal-Subcortical Protein Expression following Prenatal Exposure to Maternal Inflammation. PLoS One 6:e16638 (2011). WB; Mouse.Read more (PubMed: 21347362) »
- Sørensen BSet al. Proteins upregulated by mild and severe hypoxia in squamous cell carcinomas in vitro identified by proteomics. Radiother Oncol 92:443-9 (2009). WB; Human.Read more (PubMed: 19541378) »
See all 3 publications for this product
Publishing research using ab49343? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"