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Products:Signal Transduction >> Metabolism >> Energy Metabolism
Overview
Product name
Anti-Non Neuronal Enolase antibody
See all Non Neuronal Enolase products (8) ...
Description
Mouse monoclonal to Non Neuronal Enolase
Tested applications
WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein, corresponding to amino acids 1-435 of Human Non Neuronal Enolase
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Co-factors
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Signal Transduction >> Metabolism >> Energy Metabolism
Applications
Show applications key
Our Abpromise guarantee covers the use of ab54979 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 47 kDa.
IHC-P: Use a concentration of 5 µg/ml
ICC/IF: Use a concentration of 1 µg/ml
Target
Function
Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
Tissue specificity
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
Sequence similarities
Belongs to the enolase family.
Developmental stage
During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
Post-translational
modifications
ISGylated.
Cellular localization
Nucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
Target information above from: UniProt accessionP06733
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 2-phospho-D-glycerate hydro-lyase antibody
- Alpha-enolase antibody
- bh3 interacting domain death agonist antibody
see all
Database links
- Entrez Gene: 2023 Human
- Omim: 172430 Human
- SwissProt: P06733 Human
- Unigene: 517145 Human
Anti-Non Neuronal Enolase antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Non Neuronal Enolase antibody (ab54979)
Non Neuronal Enolase antibody (ab54979) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human lymphoma tissue.
Western blot - Non Neuronal Enolase antibody (ab54979)
Predicted band size : 47 kDa
Non Neuronal Enolase antibody (ab54979) at 1ug/lane + MCF-7 cell lysate at 25ug/lane.
Immunocytochemistry/ Immunofluorescence-Non Neuronal Enolase antibody(ab54979)
ICC/IF image of ab54979 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54979, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Non Neuronal Enolase antibody (ab54979)
This product has been referenced in:
- Soh MAet al. Arsenic, cadmium and neuron specific enolase (ENO2, ?-enolase) expression in breast cancer. Cancer Cell Int 11:41 (2011). IHC-P; Human.Read more (PubMed: 22098917) »
See 1 publication for this product
Publishing research using ab54979? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"