Anti-Notch 2 antibody (ab99404)

Overview

• Product nameAnti-Notch 2 antibodySee all Notch 2 primary antibodies ...
• Description
  Rabbit polyclonal to Notch 2
• Tested applicationsIHC-P more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Corresponds to a region between amino acid residues 2421 and 2471 of Human Notch 2 (NP_077719.2).

• Positive controlHuman breast or prostate carcinoma tissue

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: 0.1% BSA, Tris buffered saline
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Neural Stem Cells
  • Surface Molecules

  • Stem Cells
  • Signaling Pathways
  • Notch
  • Nuclear

  • Stem Cells
  • Signaling Pathways
  • Notch
  • Surface Molecules

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

  • Neuroscience
  • Neurology process
  • Notch Pathway

Applications

Target

• FunctionFunctions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation.
• Tissue specificityExpressed in the brain, heart, kidney, lung, skeletal muscle and liver. Ubiquitously expressed in the embryo.
• Involvement in diseaseAlagille syndrome 2 (ALGS2) [MIM:610205]: A form of Alagille syndrome, an autosomal dominant multisystem disorder. It is clinically defined by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems. Note=The disease is caused by mutations affecting the gene represented in this entry.
  Hajdu-Cheney syndrome (HJCYS) [MIM:102500]: A rare skeletal disorder characterized by the association of facial anomalies, acro-osteolysis, general osteoporosis, insufficient ossification of the skull, and periodontal disease (premature loss of permanent teeth). Other features include cleft palate, congenital heart defects, polycystic kidneys, orthopedic problems and anomalies of the genitalia, intestines and eyes. Note=The disease is caused by mutations affecting the gene represented in this entry. NOTCH2 mutations associated with Hajdu-Cheney syndrome cluster to the last coding exon of the gene. This suggests that the mutant mRNA products may escape nonsense-mediated decay and the resulting truncated NOTCH2 proteins act in a gain-of-function manner.
• Sequence similaritiesBelongs to the NOTCH family.
  Contains 6 ANK repeats.
  Contains 35 EGF-like domains.
  Contains 3 LNR (Lin/Notch) repeats.
• Post-translational
  modificationsSynthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
  Hydroxylated by HIF1AN.
• Cellular localizationCell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.

Target information above from: UniProt accession Q04721 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4853 Human
  • Omim: 600275 Human
  • SwissProt: Q04721 Human
  • Unigene: 487360 Human

• Alternative names
  AGS2 antibodyhN2 antibodyMotch B antibody

  N2 antibodyneurogenic locus notch homolog protein 2 antibodyNOTC2_HUMAN antibodyNotch 2 antibodyNotch 2 intracellular domain antibodyNotch Drosophila homolog 2 antibodyNotch homolog 2 antibodyNotch homolog 2 Drosophila antibodyNotch2 antibody

  see all

Anti-Notch 2 antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Notch 2 antibody (ab99404)
  ab99404, at a 1/250 dilution, staining Human Notch 2 in breast carcinoma tissue by Immunohistochemistry. Formalin/PFA-fixed paraffin-embedded tissue using DAB detection.