Notch1 antibody - Cleaved - Val1744 (ab52301)

Product Name 

Notch1 antibody - Cleaved - Val1744

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Notch1 - Cleaved - Val1744

Immunogen 

A synthetic peptide derived from human Notch 1.

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

ab52301 detects endogenous levels of fragment of activated Notch 1 resulting from cleavage adjacent to Val1744.

Tested applications 
(see key)

ELISA, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/10000.
IHC-P: Use at an assay dependent dilution (PMID : 20056840).
WB: 1/500 - 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 272 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Extracts from NIH 3T3 cells, treated with etoposide (25µM, 1hour).

Cellular localization 

Cell Membrane and Nuclear when activated

Research areas 

Developmental Biology >> Embryogenesis >> Somatogenesis
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules

Stem Cells >> Signaling Pathways >> Notch >> Surface Molecules
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Notch Pathway

Relevance 

The Notch gene belongs to a family of epidermal growth factor (EGF) like homeotic genes, which encode transmembrane proteins with a variable number of cysteine rich EGF like repeats in the extracellular region. Four notch genes have been described in mammals: Notch1, Notch2, Notch3 and Notch4 (Int3), which have been implicated in the differentiation of the nervous system and other structures. The EGF like proteins Delta and Serrate have been identified as ligands of Notch1. Mature Notch proteins are heterodimeric receptors derived from the cleavage of Notch pre-proteins into an extracellular subunit (NEC) containing multiple EGF like repeats and a transmembrane subunit including intracellular region (Ntm).

The Notch signaling pathway was first identified in Drosophila. The Notch 1 protein is a single pass transmembrane receptor activated through the Delta (Dl) and Serrate (Ser) ligands in Drosophila (and their mammalian counterparts Delta like 3 and Jagged 1 and 2). Activation results in the proteolytic cleavage of the cytoplasmic portion of Notch (120kDa) which localizes to the nucleus to interact with DNA binding proteins (RB Jk/KBF2/CBF1). The interaction of Notch with these proteins converts them from transcriptional repressors to activators, thus affecting the expression of a variety of target genes. During maturation, the Notch molecule is cleaved by furin-like convertase at the extracellular domain. Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the C terminal Notch 1 fragment dissociates from its N terminal counterpart and is further cleaved between Gly1743 and Val1744. The resulting activated cytosolic fragment translocates to the nucleus and activates Notch related transcription.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

ab52301 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

Notes 

ab52301 is cleaved at Val 1744

See below for Notch1 antibody - Cleaved - Val1744 images, references, products related to ab52301 and other tools.

Notch1 antibody - Cleaved - Val1744 images:

References for Notch1 antibody - Cleaved - Val1744 (ab52301)

  This product has been used in:

Zhu F et al. Preventive effect of Notch signaling inhibition by a gamma-secretase inhibitor on peritoneal dialysis fluid-induced peritoneal fibrosis in rats. Am J Pathol 176:650-9 (2010). WB, IHC-P; Rat. PubMed: 20056840

If you publish research using ab52301 please let us know so that we can cite the reference on this datasheet.

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