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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab52627? Please let us know so that we can cite the reference in this datasheet
ab52627 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Western blot - Notch1 antibody [EP1238Y] (ab52627)](/ps/datasheet/Images/52/ab52627/ab52627_WB_1.jpg)
Western blot - Notch1 antibody [EP1238Y] (ab52627)
Anti-Notch1 antibody [EP1238Y] (ab52627) at 1/500 dilution + Hek293 cell lysate at 10 µg
Secondary
goat anti-rabbit HRP at 1/2000 dilution
Observed band size : 125 kDa (why is the actual band size different from the predicted?)
![Immunohistochemistry (Paraffin-embedded sections) - Notch1 antibody [EP1238Y] (ab52627)](/ps/datasheet/Images/52/ab52627/ab52627_IHC_1.jpg)
Immunohistochemistry (Paraffin-embedded sections) - Notch1 antibody [EP1238Y] (ab52627)
ab52627, at a 1/50 dilution, staining human Notch1 in fetal lung tissue, using Immunohistochemistry, Paraffin embedded tissue.
](/ps/datasheet/images/52/ab52627/Notch1-Primary-antibodies-ab52627-1.jpg)
Immunocytochemistry/ Immunofluorescence-Notch1 antibody [EP1238Y](ab52627)
ICC/IF image of ab52627 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52627, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Notch1 antibody [EP1238Y] for Western blot in Mouse (52627)](/ps/datasheet/images/52/ab52627/Notch1-Primary-antibodies-ab52627-3.jpg)
Notch1 antibody [EP1238Y] for Western blot in Mouse (52627)
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