Notch1 antibody [bTAN 20] (ab79972)

Product Name 

Notch1 antibody [bTAN 20]

Product type 

Primary antibodies

Description 

Rat monoclonal [bTAN 20] to Notch1

Immunogen 

Fusion protein, corresponding to intracellular nucleotides 6658-7131 of Human Notch1 fused to proprietary protein.

Reacts with 
(species key)

Hu, Ms, Dog

Tested applications 
(see key)

ICC/IF, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 300 kDa (predicted molecular weight: 272 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Cervical squamous carcinoma and adenocarcinoma tissues; BHK cells transfected with Notch1.

Cellular localization 

Cell Membrane and Nuclear when activated

Research areas 

Developmental Biology >> Embryogenesis >> Somatogenesis
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules

Stem Cells >> Signaling Pathways >> Notch >> Surface Molecules
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Notch Pathway

Relevance 

The Notch gene belongs to a family of epidermal growth factor (EGF) like homeotic genes, which encode transmembrane proteins with a variable number of cysteine rich EGF like repeats in the extracellular region. Four notch genes have been described in mammals: Notch1, Notch2, Notch3 and Notch4 (Int3), which have been implicated in the differentiation of the nervous system and other structures. The EGF like proteins Delta and Serrate have been identified as ligands of Notch1. Mature Notch proteins are heterodimeric receptors derived from the cleavage of Notch pre-proteins into an extracellular subunit (NEC) containing multiple EGF like repeats and a transmembrane subunit including intracellular region (Ntm).

The Notch signaling pathway was first identified in Drosophila. The Notch 1 protein is a single pass transmembrane receptor activated through the Delta (Dl) and Serrate (Ser) ligands in Drosophila (and their mammalian counterparts Delta like 3 and Jagged 1 and 2). Activation results in the proteolytic cleavage of the cytoplasmic portion of Notch (120kDa) which localizes to the nucleus to interact with DNA binding proteins (RB Jk/KBF2/CBF1). The interaction of Notch with these proteins converts them from transcriptional repressors to activators, thus affecting the expression of a variety of target genes. During maturation, the Notch molecule is cleaved by furin-like convertase at the extracellular domain. Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the C terminal Notch 1 fragment dissociates from its N terminal counterpart and is further cleaved between Gly1743 and Val1744. The resulting activated cytosolic fragment translocates to the nucleus and activates Notch related transcription.

Database links 

Raised in 

Rat

Clonality 

Monoclonal

Clone number 

bTAN 20

Isotype 

IgG1

Light chain type 

kappa

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

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