Anti-Nrf2 antibody [EP1808Y] - Carboxyterminal end (ab62352)

  Western blot - Nrf2 antibody [EP1808Y] - Carboxyterminal end (ab62352)

Anti-Nrf2 antibody [EP1808Y] - Carboxyterminal end (ab62352) at 1/2000 dilution + HepG2 cell lysate at 10 µg

Secondary
goat anti rabbit HRP at 1/2000 dilution

Predicted band size : 68 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nrf2 antibody [EP1808Y] - Carboxyterminal end (ab62352)

Immunohistochemical analysis of Nrf2 expression in paraffin embedded human lung adenocarcinoma using 1/100 ab62352

  Immunocytochemistry/ Immunofluorescence - Nrf2 antibody [EP1808Y] - Carboxyterminal end (ab62352)

ICC/IF image of ab62352 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62352, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  Immunocytochemistry/ Immunofluorescence - Nrf2 antibody [EP1808Y] - Carboxyterminal end (ab62352)

ICC/IF image of ab62352 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62352, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.