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Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Overview
Product name
Anti-Nrf2 antibody
See all Nrf2 products (12) ...
Description
Rabbit polyclonal to Nrf2
Specificity
ab31163 recognises Nrf2 protein
Tested applications
IHC-P, ICC/IF, IHC-Fr, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Chicken, Cow
Immunogen
Synthetic peptide: TL YLEVFSMLRD EDGKPYSP, corresponding to amino acids 569-588 of Human Nrf2
TL YLEVFSMLRD EDGKPYSP
Positive control
Placenta
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Other Antibodies >> Oxidative Stress
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab31163 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC/IF: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution. (PubMed: 17142801)
WB: 1/1000Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).(See Abreview.)
Target
Function
Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
Tissue specificity
Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
Sequence similarities
Belongs to the bZIP family. CNC subfamily.
Contains 1 bZIP domain.
Domain
Acidic activation domain in the N-terminus, and DNA binding domain in the C-terminus.
Post-translational
modifications
Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.
Cellular localization
Cytoplasm > cytosol. Nucleus. Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.
Target information above from: UniProt accessionQ16236
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- erythroid derived 2 antibody
- like 2 antibody
- HEBP1 antibody
see all
Database links
- Entrez Gene: 396014 Chicken
- Entrez Gene: 497024 Cow
- Entrez Gene: 4780 Human
- Entrez Gene: 18024 Mouse
- Entrez Gene: 83619 Rat
- Omim: 600492 Human
- SwissProt: Q16236 Human
- SwissProt: Q60795 Mouse
see all
Anti-Nrf2 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nrf2 antibody (ab31163)
ab31163 at 1/100 dilution, staining Nrf2 in human placenta by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).
Immunocytochemistry/ Immunofluorescence-Nrf2 antibody(ab31163)
ICC/IF image of ab31163 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31163, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Nrf2 antibody (ab31163)
All lanes : Anti-Nrf2 antibody (ab31163) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 68 kDa
Observed band size : 68 kDa
Additional bands at : 28 kDa,37 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 1 minute
References for Anti-Nrf2 antibody (ab31163)
This product has been referenced in:
- Pendyala Set al. Nrf2 regulates hyperoxia-induced Nox4 expression in human lung endothelium: Identification of functional antioxidant response elements on the Nox4 promoter. Free Radic Biol Med : (2011). WB, ICC/IF; Human, Mouse.Read more (PubMed: 21443946) »
- Ungvari Zet al. Age-Associated Vascular Oxidative Stress, Nrf2 Dysfunction, and NF-{kappa}B Activation in the Nonhuman Primate Macaca mulatta. J Gerontol A Biol Sci Med Sci : (2011).Read more (PubMed: 21622983) »
See all 9 publications for this product
Publishing research using ab31163? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"