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ab53019 has been referenced in 4 publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-Nrf2 antibody (ab53019) at 1/500 dilution
Lane 1 : Extracts from HuvEc cells with no immunizing peptide
Lane 2 : Extracts from HuvEc cells with immunizing peptide
Predicted band size : 68 kDa
Observed band size : 68 kDa
ab53019 (1/100) staining Nrf2 in paraffin-embedded human lung carcinoma tissue. Right hand panel represents a negative control for which ab53019 was pre-incubated with the immunizing (blocking) peptide.
ICC/IF image of ab53019 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53019, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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