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Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
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Proteins and Peptides
ab133404
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Secondary antibodies
- Lysates
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab76026 for help.
Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.
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immunogen peptide available for sale? |
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ANSWER: |
Thank you so much for your patience. |
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Why is this antibody listed as non-reactive with mouse and rat? Do you have any data to support this? |
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ANSWER: |
Thank you for contacting us. This antibody was tested on mouse and rat brain, heart, kidney, and spleen lysates. A very faint band was detected at 100 kDa in mouse kidney and rat brain, but the other lanes showed no signal. Please see the attached image for details. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Western blot - Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)](/ps/datasheet/Images/76/ab76026/nrf1.jpg)
Western blot - Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
All lanes : Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026) at 1/20000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HepG2 cell lysate treated with AP
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/1000 dilution
Predicted band size : 68 kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)](/ps/datasheet/images/76/ab76026/Nrf2-Primary-antibodies-ab76026-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab76026 at 1/100 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)](/ps/datasheet/images/76/ab76026/Nrf2-Primary-antibodies-ab76026-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
ab76026 staining Nrf2 (phospho S40) in Human normal lung tissue sections by IHC-P (Formaldehyde-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% casein for 30 minutes at 4ºC. Antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50) in 1% casein for 24 hours at 4ºC. An undiluted HRP-conjugated Goat polyclonal to rabbit IgG was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
](/ps/datasheet/images/76/ab76026/Nrf2-Primary-antibodies-ab76026-5.jpg)
Immunocytochemistry/ Immunofluorescence-Nrf2 (phospho S40) antibody [EP1809Y](ab76026)
ICC/IF image of ab76026 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76026, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
](/ps/datasheet/images/76/ab76026/Nrf2-Primary-antibodies-ab76026-7.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Nrf2 (phospho S40) antibody [EP1809Y](ab76026)
ab76026 showing positive staining in Breast carcinoma tissue.
](/ps/datasheet/images/76/ab76026/Nrf2-Primary-antibodies-ab76026-8.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Nrf2 (phospho S40) antibody [EP1809Y](ab76026)
ab76026 showing positive staining in Cervical carcinoma tissue.
](/ps/datasheet/images/76/ab76026/Nrf2-Primary-antibodies-ab76026-9.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Nrf2 (phospho S40) antibody [EP1809Y](ab76026)
ab76026 showing positive staining in Ovarian carcinoma tissue.
](/ps/datasheet/images/76/ab76026/Nrf2-Primary-antibodies-ab76026-10.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Nrf2 (phospho S40) antibody [EP1809Y](ab76026)
ab76026 showing positive staining in Normal tonsil tissue.
![Nrf2 (phospho S40) antibody [EP1809Y] for WB in Human (76026)](/ps/datasheet/images/76/ab76026/Nrf2-Primary-antibodies-ab76026-4.jpg)
Nrf2 (phospho S40) antibody [EP1809Y] for WB in Human (76026)
![Nrf2 (phospho S40) antibody [EP1809Y] for IHC-P in Human (76026)](/ps/datasheet/images/76/ab76026/Nrf2-Primary-antibodies-ab76026-2.jpg)
Nrf2 (phospho S40) antibody [EP1809Y] for IHC-P in Human (76026)
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