![Western blot - Nrf2 (phospho S40) antibody [EP1809Y] (ab76026) image](#nrf1.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nrf2 (phospho S40) antibody [EP1809Y] (ab76026) image](#Nrf2-Primary-antibodies-ab76026-1.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nrf2 (phospho S40) antibody [EP1809Y] (ab76026) image](#Nrf2-Primary-antibodies-ab76026-3.jpg)
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Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Overview
Product name
Anti-Nrf2 (phospho S40) antibody [EP1809Y]
See all Nrf2 products (12) ...
Description
Rabbit monoclonal [EP1809Y] to Nrf2 (phospho S40)
Tested applications
ICC/IF, IHC-P, WB, IP, ICCmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
Synthetic phospho-peptide (Human) corresponding to residues near serine 40 of Nrf2.
Positive control
Human breast carcinoma tissue HepG2 cell lysate
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP1809Y
Isotype
IgG
Research areas
Cell Biology >> Other Antibodies >> Oxidative Stress
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
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Western blot - Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
![Western blot - Nrf2 (phospho S40) antibody [EP1809Y] (ab76026) image](/ps/datasheet/Images/76/ab76026/nrf1.jpg)
(enlarge)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nrf2 (phospho S40) antibody [EP1809Y] (ab76026) image](/ps/datasheet/images/76/ab76026/Nrf2-Primary-antibodies-ab76026-1.jpg)
(enlarge)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nrf2 (phospho S40) antibody [EP1809Y] (ab76026) image](/ps/datasheet/images/76/ab76026/Nrf2-Primary-antibodies-ab76026-3.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab76026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
IHC-P: 1/100 - 1/250.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: 1/5000 - 1/50000.Predicted molecular weight: 68 kDa.
IP
IP: 1/40IP: 1/40
ICC
ICC: 1/100 - 1/250.ICC: 1/100 - 1/250.
Application notes
Is unsuitable for or Flow Cyt.
Target
Function
Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
Tissue specificity
Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
Sequence similarities
Belongs to the bZIP family. CNC subfamily.
Contains 1 bZIP domain.
Domain
Acidic activation domain in the N-terminus, and DNA binding domain in the C-terminus.
Post-translational
modifications
Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.
Cellular localization
Cytoplasm > cytosol. Nucleus. Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.
Target information above from: UniProt accessionQ16236
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- erythroid derived 2 antibody
- like 2 antibody
- HEBP1 antibody
see all
Database links
- Entrez Gene: 4780 Human
- Omim: 600492 Human
- SwissProt: Q16236 Human
Anti-Nrf2 (phospho S40) antibody [EP1809Y] images:
Western blot - Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
All lanes : Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026) at 1/20000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HepG2 cell lysate treated with AP
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/1000 dilution
Predicted band size : 68 kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab76026 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
ab76026 staining Nrf2 (phospho S40) in Human normal lung tissue sections by IHC-P (Formaldehyde-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% casein for 30 minutes at 4°C. Antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50) in 1% casein for 24 hours at 4°C. An undiluted HRP-conjugated Goat polyclonal to rabbit IgG was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
Immunocytochemistry/ Immunofluorescence-Nrf2 (phospho S40) antibody [EP1809Y](ab76026)
](/ps/datasheet/images/76/ab76026/Nrf2-Primary-antibodies-ab76026-5.jpg)
ICC/IF image of ab76026 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76026, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
This product has been referenced in:
- Murakami Get al. Chemical library screening identifies a small molecule that downregulates SOD1 transcription for drugs to treat amyotrophic lateral sclerosis. J Biomol Screen 16:405-14 (2011). WB.Read more (PubMed: 21364087) »
- Niture SKet al. Antioxidant-induced modification of INrf2 cysteine 151 and PKC-delta-mediated phosphorylation of Nrf2 serine 40 are both required for stabilization and nuclear translocation of Nrf2 and increased drug resistance. J Cell Sci 122:4452-64 (2009). WB; Mouse.Read more (PubMed: 19920073) »
See all 2 publications for this product
Publishing research using ab76026? Please let us know so that we can cite the reference in this datasheet
![Nrf2 (phospho S40) antibody [EP1809Y] for WB in Human (76026)](/ps/datasheet/images/76/ab76026/Nrf2-Primary-antibodies-ab76026-4.jpg)
![Nrf2 (phospho S40) antibody [EP1809Y] for IHC-P in Human (76026)](/ps/datasheet/images/76/ab76026/Nrf2-Primary-antibodies-ab76026-2.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"