Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)

Overview

• Product nameAnti-Nrf2 (phospho S40) antibody [EP1809Y]See all Nrf2 primary antibodies ...
• Description
  Rabbit monoclonal [EP1809Y] to Nrf2 (phospho S40)
• Tested applicationsICC/IF, IHC-P, WB, IP, ICC, Flow Cyt more details
• Species reactivity
  Reacts with: Human
  

  Does not react with

  Mouse, Rat
• Immunogen

  Synthetic phospho-peptide (Human) corresponding to residues near serine 40 of Nrf2.

• Positive controlHuman breast carcinoma tissue, HepG2 cell lysate
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEP1809Y
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial Biogenesis

  • Metabolism
  • Pathways and Processes
  • Redox metabolism
  • Oxidative stress

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Nucleotide metabolism
  • Molecular processes
  • Mitochondrial transcription

  • Cardiovascular
  • Heart
  • Cardiac metabolism

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

  • Cell Biology
  • Other Antibodies
  • Oxidative Stress

Applications

Target

• FunctionTranscription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
• Tissue specificityWidely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
• Sequence similaritiesBelongs to the bZIP family. CNC subfamily.
  Contains 1 bZIP domain.
• DomainAcidic activation domain in the N-terminus, and DNA binding domain in the C-terminus.
• Post-translational
  modificationsPhosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.
• Cellular localizationCytoplasm > cytosol. Nucleus. Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.

Target information above from: UniProt accession Q16236 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4780 Human
  • Omim: 600492 Human
  • SwissProt: Q16236 Human

• Alternative names
  erythroid derived 2 antibodyHEBP1 antibodylike 2 antibody

  NF E2 related factor 2 antibodyNF-E2-related factor 2 antibodyNF2L2_HUMAN antibodyNFE2 related factor 2 antibodyNFE2-related factor 2 antibodyNFE2L2 antibodyNrf 2 antibodyNRF2 antibodyNuclear factor antibodyNuclear factor erythroid 2 related factor 2 antibodyNuclear factor erythroid 2-related factor 2 antibodyNuclear factor erythroid derived 2 like 2 antibody

  see all

Anti-Nrf2 (phospho S40) antibody [EP1809Y] images

• 

  Flow Cytometry - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  Overlay histogram showing Jurkat cells stained with ab76026 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76026, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
• 

  Western blot - Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  All lanes : Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026) at 1/20000 dilution
  
  Lane 1 : HepG2 cell lysate
  Lane 2 : HepG2 cell lysate treated with AP
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP labelled goat anti-rabbit at 1/1000 dilution
  
  Predicted band size : 68 kDa
  Observed band size : 90 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab76026 at 1/100 dilution.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)This image is courtesy of an anonymous Abreview
  ab76026 staining Nrf2 (phospho S40) in Human normal lung tissue sections by IHC-P (Formaldehyde-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% casein for 30 minutes at 4°C. Antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50) in 1% casein for 24 hours at 4°C. An undiluted HRP-conjugated Goat polyclonal to rabbit IgG was used as the secondary antibody.

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence-Nrf2 (phospho S40) antibody [EP1809Y](ab76026)
  ICC/IF image of ab76026 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76026, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Nrf2 (phospho S40) antibody [EP1809Y](ab76026)
  ab76026 showing positive staining in Breast carcinoma tissue.
  
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Nrf2 (phospho S40) antibody [EP1809Y](ab76026)
  ab76026 showing positive staining in Cervical carcinoma tissue.
  
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Nrf2 (phospho S40) antibody [EP1809Y](ab76026)
  ab76026 showing positive staining in Ovarian carcinoma tissue.
  
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Nrf2 (phospho S40) antibody [EP1809Y](ab76026)
  ab76026 showing positive staining in Normal tonsil tissue.