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Read our guarantee »Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Anti-Nrf2 (phospho S40) antibody [EP1809Y]
See all Nrf2 products (12) ...
Rabbit monoclonal [EP1809Y] to Nrf2 (phospho S40)
ICC/IF, IHC-P, WB, IP, ICCmore details
Reacts with
Human
Does not react with
Mouse, Rat
Synthetic phospho-peptide (Human) corresponding to residues near serine 40 of Nrf2.
Human breast carcinoma tissue HepG2 cell lysate
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Tissue culture supernatant
Monoclonal
EP1809Y
IgG
Cell Biology >> Other Antibodies >> Oxidative Stress
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Western blot - Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
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Our Abpromise guarantee covers the use of ab76026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
IHC-P: 1/100 - 1/250.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: 1/5000 - 1/50000.Predicted molecular weight: 68 kDa.
IP: 1/40
ICC: 1/100 - 1/250.
Is unsuitable for or Flow Cyt.
Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
Belongs to the bZIP family. CNC subfamily.
Contains 1 bZIP domain.
Acidic activation domain in the N-terminus, and DNA binding domain in the C-terminus.
Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.
Cytoplasm > cytosol. Nucleus. Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.
Target information above from: UniProt accessionQ16236
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
![Western blot - Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)](/ps/datasheet/Images/76/ab76026/nrf1.jpg)
All lanes : Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026) at 1/20000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HepG2 cell lysate treated with AP
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/1000 dilution
Predicted band size : 68 kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)](/ps/datasheet/images/76/ab76026/Nrf2-Primary-antibodies-ab76026-1.jpg)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab76026 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)](/ps/datasheet/images/76/ab76026/Nrf2-Primary-antibodies-ab76026-3.jpg)
ab76026 staining Nrf2 (phospho S40) in Human normal lung tissue sections by IHC-P (Formaldehyde-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% casein for 30 minutes at 4°C. Antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50) in 1% casein for 24 hours at 4°C. An undiluted HRP-conjugated Goat polyclonal to rabbit IgG was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
Immunocytochemistry/ Immunofluorescence-Nrf2 (phospho S40) antibody [EP1809Y](ab76026)
](/ps/datasheet/images/76/ab76026/Nrf2-Primary-antibodies-ab76026-5.jpg)
ICC/IF image of ab76026 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76026, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
See all 2 publications for this product
Publishing research using ab76026? Please let us know so that we can cite the reference in this datasheet
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![Western blot - Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)](/ps/datasheet/Images/76/ab76026/nrf1.jpg)
All lanes : Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026) at 1/20000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HepG2 cell lysate treated with AP
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/1000 dilution
Predicted band size : 68 kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)](/ps/datasheet/images/76/ab76026/Nrf2-Primary-antibodies-ab76026-1.jpg)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab76026 at 1/100 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)](/ps/datasheet/images/76/ab76026/Nrf2-Primary-antibodies-ab76026-3.jpg)
ab76026 staining Nrf2 (phospho S40) in Human normal lung tissue sections by IHC-P (Formaldehyde-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% casein for 30 minutes at 4°C. Antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50) in 1% casein for 24 hours at 4°C. An undiluted HRP-conjugated Goat polyclonal to rabbit IgG was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
](/ps/datasheet/images/76/ab76026/Nrf2-Primary-antibodies-ab76026-5.jpg)
ICC/IF image of ab76026 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76026, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
2
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