Anti-Nsp1p antibody [32D6 ] - Nuclear Envelope and Nuclear Pore Marker (ab4641)

Overview

• Product nameAnti-Nsp1p antibody [32D6 ] - Nuclear Envelope and Nuclear Pore Marker
• Description
  Mouse monoclonal [32D6 ] to Nsp1p - Nuclear Envelope and Nuclear Pore Marker
• SpecificityThis antibody was initially identified as directed against a nuclear pore complex antigen by immunofluorescence localization. A screen of a lgt11 expression library yielded a single positive clone carrying an insert bearing ~66% of the C-terminal portion of NSP1. To confirm that 32D6 possessed high affinity for Nsp1p, strain RS453, which expresses a shortened isoform of Nsp1p, was compared to the wildtype strain BJ5465. NSP1 in RS453 contains an internal deletion that removes the coding sequence for 6 FXFG repeats, which are not essential for function and encodes a protein that has been observed to migrate at approxmately 85 kDa on SDS gels. The predicted size of wildtype Nsp1p is 86.5 kDa, but Nsp1p has been observed to migrate on SDS gels at ~100 kDa. In our gels, the apparent molecular mass of wildtype Nsp1p was 108 kDa, whereas the short isoform of Nsp1p in RS453 migrated at 91 kDa. The detection of two protein bands of apparent sizes 108 kDa and 91 kDa in BJ5465 and RS453, respectively, demonstrated that 32D6 recognized the pore complex protein Nsp1p.
• Tested applicationsICC/IF, WB more details
• Species reactivity
  Reacts with: Saccharomyces cerevisiae
  
• Immunogen

  This was raised against a yeast nucleolar prep, and screened by immunocytochemistry and western blotting.

• General notes

  
  

  This antibody is a useful marker of yeast nuclear pores.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 10mM Sodium Azide
  Constituents: Tissue Culture Supernatant
• PurityTissue culture supernatant
• Purification notesSterile filtered.
• Primary antibody notes This antibody is a useful marker of yeast nuclear pores.
• Clonality Monoclonal
• Clone number32D6
• IsotypeIgG
• Research Areas
  • Tags & Cell Markers
  • Subcellular Markers
  • Nucleus
  • Nuclear Envelope

  • Tags & Cell Markers
  • Subcellular Markers
  • Nucleus
  • Nuclear Pore

Applications

Target

• RelevanceNSP1 functions as a component of the nuclear pore complex (NPC). It plays an important role in several nuclear transport pathways including poly(A)+ RNA, tRNA, pre-ribosome, signal recognition particle (SRP), and protein transport.
• Cellular localizationNuclear membrane: part of the nuclear pore complex, it presents a symmetric distribution with a cytoplasmic and a nucleoplasmic side.
• Database links
  • Entrez Gene: 853409 Saccharomyces cerevisiae
  • SwissProt: P14907 Saccharomyces cerevisiae

• Alternative names
  NSP 1 antibodyNSP1 antibodyNsp1p antibody

  Nuclear pore protein NSP1 antibodyNucleoporin NSP1 antibodyNucleoskeletal-like protein antibodyp110 antibody

  see all

Anti-Nsp1p antibody [32D6 ] - Nuclear Envelope and Nuclear Pore Marker images

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  Immunocytochemistry/ Immunofluorescence - Nsp1p antibody [32D6 ] - Nuclear Envelope and Nuclear Pore Marker (ab4641)Image courtesy of an anonymous Abreview.
  ab4641 (1/5000) detecting Nsp1p in Saccharomyces cerevisae DDY1810 (green). Cells were fixed in paraformaldehyde, permeabilized in methanol/acetone and counterstained with DAPI in order to highlight the nucleus (blue). For further experimental details please refer to Abreview.

  See Abreview