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Product Name
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NuMA antibody [A73-B/D12]
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See all NuMA antibodies (5)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [A73-B/D12] to NuMA
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Immunogen
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Live Ls 174T cells (human colon carcinoma).
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Reacts with
(species key)
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Hu
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Specificity
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Reacts with an intracellular 228 kD protein found in the nucleus during interphase.
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Tested applications
(see key)
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IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: 1/50 - 1/100.
WB: 1/100 - 1/300. Detects a band of approximately 228 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Tonsil, Thymus, Spleen.
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Cellular localization
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Nuclear
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Research areas
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Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Cell Biology >> Cell Cycle >> Markers
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Relevance
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NuMA (Nuclear Mitotic Apparatus Protein) is an intranuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200kDa during apoptosis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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A73-B/D12
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Isotype
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IgM
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Purity
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Purified IgM
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: Foetal Calf Serum, Carrier protein, Dialysed media, 20mM Tris-borate, 150mM Sodium Chloride. pH 7.5 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.300 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this NuMA antibody [A73-B/D12] is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to NuMA
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NuMA antibody [A73-B/D12] - more information
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