Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cell Biology >> Cell Cycle >> Markers
Overview
Product name
Anti-NuMA antibody
See all NuMA products (8) ...
Description
Rabbit polyclonal to NuMA
Tested applications
ICC/IF, WB, IFmore details
Cross reactivity
Reacts with
Mouse, Chicken, Human
Predicted to work with
all Mammals
Immunogen
Recombinant full length protein (Human) expressed in insect Sf9 cells using baculovirus expression system, then purified to near homogeneity prior to immunization.
Positive control
HeLa whole cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Cell Biology >> Cell Cycle >> Markers
-
Immunocytochemistry/ Immunofluorescence - NuMA antibody (ab36999)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab36999 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution.
WB: 1/1000Detects a band of approximately 220 kDa (predicted molecular weight: 238 kDa).
IF
IF: 1/500IF: 1/500
Target
Function
May be a structural component of the nucleus.
Cellular localization
Nucleus. Chromosome. Dissociates from condensing chromosomes during early prophase, before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident.
Target information above from: UniProt accessionQ14980
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Centrophilin stabilizes mitotic spindle in mitotic cells antibody
- Nuclear mitotic apparatus protein 1 antibody
- Nuclear Mitotic Apparatus Protein antibody
see all
Database links
- Entrez Gene: 4926 Human
- Entrez Gene: 101706 Mouse
- Omim: 164009 Human
- SwissProt: Q14980 Human
- SwissProt: Q3TH77 Mouse
- Unigene: 325978 Human
Anti-NuMA antibody images:
Immunocytochemistry/ Immunofluorescence - NuMA antibody (ab36999)
ab36999 at 1/1000 dilution, staining human HeLa cells fixed with 3.5% formaldehyde, by immunofluorescence, showing the typical pattern for NuMa in the cell nucleus during interphase and on spindles during mitosis.
References for Anti-NuMA antibody (ab36999)
This product has been referenced in:
- Barr ARet al. CDK5RAP2 functions in centrosome to spindle pole attachment and DNA damage response. J Cell Biol 189:23-39 (2010). ICC/IF; Chicken.Read more (PubMed: 20368616) »
- Poulson ND & Lechler T Robust control of mitotic spindle orientation in the developing epidermis. J Cell Biol 191:915-22 (2010). ICC/IF; Mouse.Read more (PubMed: 21098114) »
See all 3 publications for this product
Publishing research using ab36999? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"