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Immunocytochemistry/ Immunofluorescence - NuMA antibody (ab36999)
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Product Name
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NuMA antibody
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See all NuMA antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to NuMA
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Immunogen
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Recombinant full length protein (Human) expressed in insect Sf9 cells using baculovirus expression system, then purified to near homogeneity prior to immunization.
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Reacts with
(species key)
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Mamm
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IF: 1/500.
WB: 1/1000. Detects a band of approximately 220 kDa (predicted molecular weight: 238 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa whole cells.
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Cellular localization
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Nuclear
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Research areas
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Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Cell Biology >> Cell Cycle >> Markers
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Relevance
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NuMA (Nuclear Mitotic Apparatus Protein) is an intranuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200kDa during apoptosis.
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: Whole serum Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this NuMA antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for NuMA antibody images, references, products related to ab36999 and other tools.
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NuMA antibody images:
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 Immunocytochemistry/ Immunofluorescence - NuMA antibody (ab36999)
ab36999 at 1/1000 dilution, staining human HeLa cells fixed with 3.5% formaldehyde, by immunofluorescence, showing the typical pattern for NuMa in the cell nucleus during interphase and on spindles during mitosis.
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Search PubMed (MEDLINE) for references to NuMA
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NuMA antibody - more information
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Customer reviews (feedback) regarding NuMA antibody |
Customer FAQs regarding NuMA antibody |
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Price and availability of products related to NuMA antibody |
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NuMA antibody - related products:
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NuMA antibody - other tools:
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Contact Abcam with a Technical Enquiry about NuMA antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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