Overview

  • Product nameNuclear Apoptosis Assay Kit
  • Sample type
    Adherent cells, Suspension cells
  • Species reactivity
    Reacts with: Human
  • Product overview

    ab112151 is designed to monitor cell apoptosis by measuring the apoptotic chromatin condensation. The compacted chromatin of apoptotic cells binds higher amounts of nuclear dye compared to the healthy cells. The fluorometric assay is based on the detection of the DNA contents in cells using our proprietary non-fluorescent dye that becomes strongly fluorescent upon binding to cellular DNA.

    In normal cells, Nuclear Green DCS1 Dye is not cell permeable. However, in apoptotic cells, Nuclear Green DCS1 Dye can easily get into the cells with compromised plasma membranes. Once inside the cell, the dye binds to intracellular DNA producing highly fluorescent complexes which identify the cells as non-viable cells. The staining density with Nuclear Green DCS1 Dye can be measured with a flow cytometer at Ex/Em = 490/520 nm (FL1 channel) or a fluorescence microscope (FITC filter set).


    ab112151 can be used together with our other apoptosis reagents, such as our ab112149 NIR Mitochondrial Membrane Potential Assay Kit, for multi-parametric study of cell viability and apoptosis. The kit is optimized for screening apoptosis activators and inhibitors.

    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Store ab112151 dessicated.

  • Tested applicationsFluorescence Microscopy, Functional Studies, Flow Cyt more details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab112151 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Fluorescence Microscopy Use at an assay dependent concentration.
    Functional Studies Use at an assay dependent concentration.
    Flow Cyt Use at an assay dependent concentration.

    Nuclear Apoptosis Assay Kit images

    • The increase in fluorescence intensity of ab112151 DCS1 Dye with the addition of Camptothecin in Jurkat cells. Jurkat cells were untreated (A) or treated overnightwith 20 μM camptothecin (B) in a 37 oC, 5% CO2 incubator, and then dye loaded with ab112151 DCS1 Dye for 60 minutes.

    Protocols

    References for Nuclear Apoptosis Assay Kit (ab112151)

    ab112151 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"