Products:Tags & Cell Markers >> Subcellular Markers >> Nucleus >> Nuclear Pore
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ab50008 has been referenced in 4 publications.
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Lane 1 : Anti-Nuclear Pore Complex Proteins antibody [414] (ab50008) at 1 µg/ml
Lane 2 : Negative control - Goat Anti-Mouse IgG only
Lane 1 : Extract from HeLa cells
Lane 2 : As above
Secondary
Lane 1 : Goat Anti-Mouse IgG
Predicted band size : 106 kDa
Observed band size : 150 kDa (why is the actual band size different from the predicted?)
ab50008 (1:500, 1hr/ 37oC) staining Nuclear pore complex proteins in HeLa cells (green). Cells were fixed in PHEM buffer (45 mM Hepes pH 6.9, 45 mM Pipes pH 6.9, 5 mM MgCl2, 10 mM EGTA, 4% PFA) /0,2% Triton x-100, 10 min at room Tº, then quickly wash 3 x in PBS. ) Good results are also obtained after straight PFA fix: fix in 3.7% PFA / 30 mM sucrose for 10 min at room Tº, quickly wash 3 x in PBS, then incubatein PBS/ 0.1% TritonX-100 for 5 min and further in 0.1 M glycine for 10 min.
Image kindly provided by R. Mangiacasale,M. Ciciarello and P. Lavia, Institute of. Molecular Biology and Pathology, CNR - Rome (Italy)
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