Products:Tags & Cell Markers >> Subcellular Markers >> Nucleus >> Nuclear Pore
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ab24609 has been referenced in 13 publications.
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ab24609 (1/200) staininig nuclear pore complex proteins in human RPE-1 cells (green). Cells were fixed in paraformaldehyde, permeabilised with Triton X100 and counterstained with DAPI in order to highlight the nucleus (blue). Please refer to abreview for further experimental details.
This image is courtesy of an Abreview submitted by Dr Kirk McManus
ab24609 (1/500) staining nuclear pore complex proteins in human Hela Cells (green). Cells were fixed with Paraformaldehyde/Triton X-100 (10 min in PTEMF buffer (20mM PIPES, 1mM MgCl2, 10mM EGTA, 4% PFA) /0.2% Triton-X100 at room TºC) or Methanol (6 min in Methanol -20 ºC , followed by 3 washes in 1x PBS) and counterstained with Dapi in order to highligh the nucleus (blue).
Image and protocol kindly provided by Rosamaria Mangiacasale, Marilena Ciciarello and Patrizia Lavia, Univ Rome
ab24609 (1/500) staining nuclear pore complex proteins in murine NIH/3T3 Cells (green). Cells were fixed with Paraformaldehyde/Triton X-100 (10 min in PTEMF buffer (20mM PIPES, 1mM MgCl2, 10mM EGTA, 4% PFA) /0.2% Triton-X100 at room TºC) or Methanol (6 min in Methanol -20 ºC , followed by 3 washes in 1x PBS) and counterstained with Dapi in order to highligh the nucleus (blue).
Image and protocol kindly provided by Rosamaria Mangiacasale, Marilena Ciciarello and Patrizia Lavia, Univ Rome
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