Anti-Nucleolin antibody [4E2] - ChIP Grade (ab13541)

  Immunocytochemistry/ Immunofluorescence - Nucleolin antibody [4E2] (ab13541)

ab 13541 at a 1/2000 dilution staining U2OS cells by Immunocytochemistry. The antibody was incubated with the cells for 1 hour and then detected using a Alexa-Fluor ® 594 chicken anti-mouse antibody.

This image is courtesy of an Abreview submitted by Raffaela Santoro on 16 March 2006.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nucleolin antibody [4E2] (ab13541)

ab13541 at 1µg/ml staining Nucleolin in human colon cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

  Western blot - Nucleolin antibody [4E2] (ab13541)

All lanes : Anti-Nucleolin antibody [4E2] - ChIP Grade (ab13541) at 1 µg/ml

Lane 1 : Cell lysates prepared from human HL60 cells
Lane 2 : Cell lysates prepared from human ZR-75-1 cells


Predicted band size : 100 kDa

  Flow Cytometry - Nucleolin antibody [4E2] - ChIP Grade (ab13541)

Overlay histogram showing HeLa cells stained with ab13541 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13541, 1µg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.