Anti-Nucleolin antibody [4E2] - ChIP Grade (ab13541)

Overview

  • Product nameAnti-Nucleolin antibody [4E2] - ChIP GradeSee all Nucleolin primary antibodies ...
  • Description
    Mouse monoclonal [4E2] to Nucleolin - ChIP Grade
  • Tested applicationsICC/IF, IHC-P, WB, ICC, Flow Cyt, ChIPmore details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse
  • Immunogen

    Human nucleolin protein from Raji cell extract.

  • Positive control
    • Raji cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab13541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
IHC-P Use a concentration of 1 - 10 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 100 kDa.
ICC Use a concentration of 1 - 10 µg/ml.
Flow Cyt Use 1µg for 106 cells.
ChIP Use at an assay dependent dilution. PubMed: 19615975(recombinant nucleolin binding in the HPV16 genome).

Target

  • FunctionNucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
  • Sequence similaritiesContains 4 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications
    Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
  • Cellular localizationNucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links
  • Alternative names
    • C23 antibody
    • FLJ45706 antibody
    • MS1116 antibody
    • NCL antibody
    • Nucl antibody
    • NUCL_HUMAN antibody
    • Nucleolin antibody
    • Protein C23 antibody
    see all

Anti-Nucleolin antibody [4E2] - ChIP Grade images

  • ab 13541 at a 1/2000 dilution staining U2OS cells by Immunocytochemistry. The antibody was incubated with the cells for 1 hour and then detected using a Alexa-Fluor ® 594 chicken anti-mouse antibody.

    This image is courtesy of an Abreview submitted by Raffaela Santoro on 16 March 2006.

    See Abreview

  • ab13541 at 1µg/ml staining Nucleolin in human colon cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

  • All lanes : Anti-Nucleolin antibody [4E2] - ChIP Grade (ab13541) at 1 µg/ml

    Lane 1 : Cell lysates prepared from human HL60 cells
    Lane 2 : Cell lysates prepared from human ZR-75-1 cells


    Predicted band size : 100 kDa
  • Overlay histogram showing HeLa cells stained with ab13541 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13541, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

References for Anti-Nucleolin antibody [4E2] - ChIP Grade (ab13541)

This product has been referenced in:
  • Rana R & Biegalke BJ Human cytomegalovirus UL34 early and late proteins are essential for viral replication. Viruses 6:476-88 (2014). Human . Read more (PubMed: 24476753) »
  • Wakai M  et al. A human artificial chromosome recapitulates the metabolism of native telomeres in mammalian cells. PLoS One 9:e88530 (2014). WB ; Human . Read more (PubMed: 24558398) »

See all 20 Publications for this product

Product Wall

Application ChIP
Detection step Real-time PCR
Sample Human Cell lysate - whole cell (Breast)
Specification Breast
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Username

Abcam user community

Verified customer

Submitted Jun 02 2014

Application Western blot
Loading amount 1e+006 cells
Gel Running Conditions Reduced Denaturing (12%)
Sample Human Cell lysate - whole cell (Breast)
Specification Breast
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted May 27 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Human Tissue sections (Breast)
Specification Breast
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted May 23 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (primary smooth muscle cells)
Loading amount 25 µg
Specification primary smooth muscle cells
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Yiwen Liu

Verified customer

Submitted Mar 20 2012

Merci de nous avoir contactés. L'équipe qui produit l'anticorps ab13541 m'a informé que l'épitope n'avait malheureusement pas été déterminé. Désolé de ne pas pouvoir vous donner plus d'information sur ce produit.

Thank you for contacting us. The source of the antibody ab13541 informed me that, unfortunately, the epitope has not been mapped. The only information we have is that ab13541 is directed against the Human nucleotin, reference SwisProt # P19338 (http...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (glioma cell)
Specification glioma cell
Fixative Paraformaldehyde
Permeabilization Yes - NP-40+BSA in PBS
Username

Ms. YOO MIRAN

Verified customer

Submitted May 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (prostate cancer cells)
Loading amount 20 µg
Specification prostate cancer cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Sebastiano Battaglia

Verified customer

Submitted Dec 05 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (A431 Xenograft in mouse)
Specification A431 Xenograft in mouse
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Blocking step Dako Protein Block as blocking agent for 15 minute(s) · Concentration: pre dilute
Username

Abcam user community

Verified customer

Submitted Apr 27 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (U2OS cells)
Specification U2OS cells
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10%
Username

Miss. Raffaela Santoro

Verified customer

Submitted Mar 16 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"