Anti-Nucleolin antibody [4E2] - ChIP Grade (ab13541)

Overview

• Product nameAnti-Nucleolin antibody [4E2] - ChIP GradeSee all Nucleolin primary antibodies ...
• Description
  Mouse monoclonal [4E2] to Nucleolin - ChIP Grade
• Tested applicationsICC/IF, IHC-P, WB, ICC, Flow Cyt, ChIP more details
• Species reactivity
  Reacts with: Human
  

  Does not react with

  Mouse
• Immunogen

  Human nucleolin protein from Raji cell extract.

• Positive controlRaji cell extract.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 50% Glycerol, PBS
• Concentration information loading...
• PurityProtein A purified
• Purification notesThis antibody was affinity purified.
• Clonality Monoclonal
• Clone number4E2
• IsotypeIgG1
• Research Areas
  • Tags & Cell Markers
  • Subcellular Markers
  • Nucleus
  • Nucleolus

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • Translation
  • Ribosome

  • Epigenetics and Nuclear Signaling
  • ChIP'ing antibodies
  • ChIP'ing antibodies

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Synthesis
  • Other

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • RNA Processing
  • Other

Applications

Target

• FunctionNucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
• Sequence similaritiesContains 4 RRM (RNA recognition motif) domains.
• Post-translational
  modificationsSome glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
• Cellular localizationNucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Target information above from: UniProt accession P19338 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4691 Human
  • Omim: 164035 Human
  • SwissProt: P19338 Human
  • Unigene: 79110 Human

• Alternative names
  C23 antibodyFLJ45706 antibodyMS1116 antibody

  NCL antibodyNucl antibodyNUCL_HUMAN antibodyNucleolin antibodyProtein C23 antibody

  see all

Anti-Nucleolin antibody [4E2] - ChIP Grade images

• 

  Immunocytochemistry/ Immunofluorescence - Nucleolin antibody [4E2] (ab13541)

  ab 13541 at a 1/2000 dilution staining U2OS cells by Immunocytochemistry. The antibody was incubated with the cells for 1 hour and then detected using a Alexa-Fluor ® 594 chicken anti-mouse antibody.

  This image is courtesy of an Abreview submitted by Raffaela Santoro on 16 March 2006.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nucleolin antibody [4E2] (ab13541)

  ab13541 at 1µg/ml staining Nucleolin in human colon cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

• 

  Western blot - Nucleolin antibody [4E2] (ab13541)
  All lanes : Anti-Nucleolin antibody [4E2] - ChIP Grade (ab13541) at 1 µg/ml
  
  Lane 1 : Cell lysates prepared from human HL60 cells
  Lane 2 : Cell lysates prepared from human ZR-75-1 cells
  
  
  Predicted band size : 100 kDa
  
• 

  Flow Cytometry - Nucleolin antibody [4E2] - ChIP Grade (ab13541)
  Overlay histogram showing HeLa cells stained with ab13541 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13541, 1µg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.