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Full length native protein (purified) (Rat).
Our Abpromise guarantee covers the use of ab10530 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 33 kDa).|
|ELISA||Use at an assay dependent concentration.|
|IHC||Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|ICC||Use at an assay dependent concentration.|
ab10530 at 1/250 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked with serum and incubated with the antibody for 30 minutes at 22°C. An HRP conjugated goat anti-mouse antibody was used as the secondary.
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