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Full length native protein (purified) (Rat).
Our Abpromise guarantee covers the use of ab10530 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 33 kDa).|
|ELISA||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
ab10530 at 1/250 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked with serum and incubated with the antibody for 30 minutes at 22°C. An HRP conjugated goat anti-mouse antibody was used as the secondary.
This image is courtesy of an anonymous Abreview
Western blot image of ab10530 staining whole cell lysate of U2OS cells. The gel was blocked with 5% milk for 1 hour at 21°C. The primary antibody was diluted to 2 µg/ml and incubated for 12 hours at 4°C. A HRP conjugated goat anti-mouse antibody was used as the secondary.
ab10530 staining Nucleophosmin in immortalized Human trabecular meshwork cells by Immunocytochemistry/ Immunofluorescence. Cells were PFA-fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 3% BSA for 45 minutes at room temperature. The primary antibody was diluted 1/1000 in PBS/0.3% BSA and incubated with the sample for 2 hours. The secondary antibody was Cy3®-conjugated Donkey anti-Mouse polyclonal, diluted 1/1000.
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