For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome.
Full length native protein (purified) (Rat).
Our Abpromise guarantee covers the use of ab10530 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent dilution.|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent dilution.|
|WB||Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 33 kDa).|
|ELISA||Use at an assay dependent dilution.|
|ICC||Use at an assay dependent dilution.|
ab10530 at 1/250 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked with serum and incubated with the antibody for 30 minutes at 22°C. An HRP conjugated goat anti-mouse antibody was used as the secondary.
This image is courtesy of an anonymous Abreview
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"