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Products:Cell Biology >> Apoptosis >> Nucleus >> Other
Overview
Product name
Anti-Nucleoporin p62 antibody [RL31]
See all Nucleoporin p62 products (5) ...
Description
Armenian Hamster monoclonal [RL31] to Nucleoporin p62
Specificity
Detects the nucleoporin p62 O-linked glycoprotein in the p62 complex from rat tissues.
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Rat
Immunogen
O-linked glycoprotein-enriched fraction from rat liver nuclear envelopes.
Positive control
Rat liver nuclear envelope fractions.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS with 0.05% sodium azide
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
RL31
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Nuclear Pore Complex
Signal Transduction >> Protein Trafficking >> Nuclear Import / Export
Signal Transduction >> Growth Factors/Hormones >> EGF
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> NFkB Pathway
Tags & Cell Markers >> Subcellular Markers >> Nucleus >> Nuclear Pore
Cell Biology >> Apoptosis >> Nucleus >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2736 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF
WB
Application notes
ICC/IF: 1/50. This antibody stains both the nucleoplasmic and cytoplasmic surfaces of rat's liver Nup62.
WB: 1/200. This antibody detects a single 62 kDa band in rat liver nuclear envelope fractions.
Target
Relevance
The nuclear pore complex is a structure that extends across the nuclear envelope and regulates the flow of macromolecules between the cytoplasm and the nucleus. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. Nup 62 is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. There are multiple transcript variants of this gene, however, they encode a single protein isoform. This protein undergoes post-translational modifications. It contains about 10 N-acetylglucosamine side chain sites.
Cellular localization
Central region of the nuclear pore. During mitotic cell division, it associates with the poles of the mitotic spindle.
Alternative names
- 62 kDa nucleoporin antibody
- Nuclear pore glycoprotein p62 antibody
- nucleoporin 62kD antibody
see all
Database links
References for Anti-Nucleoporin p62 antibody [RL31] (ab2736)
This product has been referenced in:
- Guan Tet al. Structural analysis of the p62 complex, an assembly of O-linked glycoproteins that localizes near the central gated channel of the nuclear pore complex. Mol Biol Cell 6:1591-603 (1995).Read more (PubMed: 8589458) »
See 1 publication for this product
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"