Anti-Nucleoporin p62/NUP62 antibody (ab96134)
Key features and details
- Rabbit polyclonal to Nucleoporin p62/NUP62
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Nucleoporin p62/NUP62 antibody
See all Nucleoporin p62/NUP62 primary antibodies -
Description
Rabbit polyclonal to Nucleoporin p62/NUP62 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Zebrafish, Rhesus monkey -
Immunogen
Recombinant fragment corresponding to Human Nucleoporin p62/NUP62 aa 294-502.
Database link: NP_057637 -
Positive control
- WB: A431, H1299, PC-12, Rat2, Neuro-2a, GL261, C8D30, NIH/3T3, BCL-1, RAW 264.7 and C2C12 whole cell lysate. ICC/IF: A431 cells. IHC-P: Mouse fore brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96134 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/1000.
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WB | (2) |
1/500 - 1/3000. Predicted molecular weight: 53 kDa.
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ICC/IF | (1) |
1/100 - 1/200.
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Notes |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 53 kDa. |
ICC/IF
1/100 - 1/200. |
Target
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Relevance
The nuclear pore complex is a structure that extends across the nuclear envelope and regulates the flow of macromolecules between the cytoplasm and the nucleus. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. Nup 62 is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. There are multiple transcript variants of this gene, however, they encode a single protein isoform. This protein undergoes post-translational modifications. It contains about 10 N-acetylglucosamine side chain sites. -
Cellular localization
Nucleus; nuclear pore complex. Cytoplasm; cytoskeleton; spindle pole. Note: Central region of the nuclear pore, within the transporter. During mitotic cell division, it associates with the poles of the mitotic spindle. -
Database links
- Entrez Gene: 23636 Human
- Entrez Gene: 18226 Mouse
- Entrez Gene: 65274 Rat
- Entrez Gene: 100426308 Rhesus monkey
- Omim: 605815 Human
- SwissProt: P37198 Human
- SwissProt: Q63850 Mouse
- SwissProt: P17955 Rat
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Alternative names
- 62 kDa nucleoporin antibody
- DKFZp547L134 antibody
- FLJ20822 antibody
see all
Images
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All lanes : Anti-Nucleoporin p62/NUP62 antibody (ab96134) at 1/1000 dilution
Lane 1 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
Lane 2 : GL261 whole cell lysate
Lane 3 : C8D30 whole cell lysate
Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 5 : BCL-1 (mouse B lymphocyte lymphoma cell line) whole cell lysate
Lane 6 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 7 : C2C12 (Mouse myoblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 53 kDa10% SDS-PAGE.
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ab96134, at a 1/200 dilution, staining Nucleoporin p62/NUP62 in paraformaldehyde fixed A431 by Immunofluorescence analysis.
Lower image: merged with DNA probe. -
All lanes : Anti-Nucleoporin p62/NUP62 antibody (ab96134) at 1/1000 dilution
Lane 1 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 2 : Rat2 (Rat fibroblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 53 kDa10% SDS-PAGE.
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Paraffin-embedded mouse fore brain tissue stained for Nucleoporin p62/NUP62 using ab96134 at 1/500 dilution in immunohistochemical analysis.
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All lanes : Anti-Nucleoporin p62/NUP62 antibody (ab96134) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 53 kDa
10% SDS PAGE -
Immunofluorescence analysis of A431 cells, staining Nucleoporin p62/NUP62 with ab96134 at 1/200 dilution, demonstrating punctate nuclear staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab96134 has been referenced in 9 publications.
- Zhao Z et al. miRNA profiling of primate cervicovaginal lavage and extracellular vesicles reveals miR-186-5p as a potential antiretroviral factor in macrophages. FEBS Open Bio 10:2021-2039 (2020). PubMed: 33017084
- Li W et al. miR-129-5p attenuates hypoxia-induced apoptosis in rat H9c2 cardiomyocytes by activating autophagy. J Gene Med 22:e3200 (2020). PubMed: 32298509
- Qiao J et al. Inhibition of HIF-1a restrains fracture healing via regulation of autophagy in a rat model. Exp Ther Med 17:1884-1890 (2019). PubMed: 30783464
- Clift D et al. A Method for the Acute and Rapid Degradation of Endogenous Proteins. Cell 171:1692-1706.e18 (2017). PubMed: 29153837
- Ouyang F et al. HMGB1 induces apoptosis and EMT in association with increased autophagy following H/R injury in cardiomyocytes. Int J Mol Med 37:679-89 (2016). WB . PubMed: 26847839
- Lund PJ et al. Global Analysis of O-GlcNAc Glycoproteins in Activated Human T Cells. J Immunol 197:3086-3098 (2016). Human . PubMed: 27655845
- Guan JJ et al. DRAM1 regulates apoptosis through increasing protein levels and lysosomal localization of BAX. Cell Death Dis 6:e1624 (2015). PubMed: 25633293
- Tojo Y et al. Hypoxia Signaling Cascade for Erythropoietin Production in Hepatocytes. Mol Cell Biol 35:2658-72 (2015). Mouse . PubMed: 26012551
- Isnard A et al. Impact of Leishmania infection on host macrophage nuclear physiology and nucleopore complex integrity. PLoS Pathog 11:e1004776 (2015). WB . PubMed: 25826301