Nudel antibody (ab25959)

Product Name 

Nudel antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Nudel

Immunogen 

Synthetic peptide (Human) - corresponding to 15 amino acids near the amino terminus of human Nudel.

Reacts with 
(species key)

Hu, Ms

Specificity 

ab25959 recognises Nudel.

Tested applications 
(see key)

ICC/IF, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at a concentration of 2 µg/ml.
WB: Use at a concentration of 0.5-1.0 µg/ml. Predicted molecular weight: 38 kDa. Can be blocked with Nudel peptide (ab39840).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Jurkat cell lysate.

Cellular localization 

Cytoplasmic

Research areas 

Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Neuroscience >> Neurology process >> Neural Signal Transduction

Relevance 

Nudel is important for normal cortical development. It is invovled in microtubule organization, nuclear translocation, and neuronal positioning in concert with various other factors (including Lis1, Pafah1b1, Pahfah1b2, dynein, dynorphin A and cdk5). Western blot analysis of mouse tissues shows abundant expression of Nudel in brain and testis, and much lower expression in heart, liver, kidney, and skeletal muscle. In fractionated rat brain, Nudel and Lis1 are both found in fractions enriched for postsynaptic density proteins. Immunostaining of embryonic day 18 mouse brain sections revealed staining of migrating neurons and thalamocortical axons of the intermediate zone of the developing cerebral cortex, as well as several other developing brain regions. The deduced protein contains 345 amino acids and has a calculated molecular mass of 38.4 kDa. It has a coiled coil motif (residues 19 to 201), followed by several potential phosphorylation sites for casein kinase II, protein kinase C or CDK5. Nudel shares about 50% identity with mouse and human NUDE proteins.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C.

See below for Nudel antibody images, references, products related to ab25959 and other tools.

Nudel antibody images:

References for Nudel antibody (ab25959)

  This product has been used in: (two most recent references)

Leliveld SR et al. Insolubility of disrupted-in-schizophrenia 1 disrupts oligomer-dependent interactions with nuclear distribution element 1 and is associated with sporadic mental disease. J Neurosci 28:3839-45 (2008). PubMed: 18400883

Alkam T et al. The extensive nitration of neurofilament light chain in the hippocampus is associated with the cognitive impairment induced by amyloid beta in mice. J Pharmacol Exp Ther : (2008). WB; Mouse. PubMed: 18621989
If you publish research using ab25959 please let us know so that we can cite the reference on this datasheet.

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