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Products:Neuroscience >> Neurology process >> Neural Signal Transduction
Overview
Product name
Anti-Nudel antibody
See all Nudel products (7) ...
Description
Rabbit polyclonal to Nudel
Specificity
ab25959 recognises Nudel.
Tested applications
IP, ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide (Human) - corresponding to 15 amino acids near the amino terminus of human Nudel.
Positive control
Jurkat cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Neuroscience >> Neurology process >> Neural Signal Transduction
Applications
Show applications key
Our Abpromise guarantee covers the use of ab25959 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution. (PubMed: 17984054)
ICC/IF: Use a concentration of 2 µg/ml
WB: Use a concentration of 0.5-1.0 µg/mlPredicted molecular weight: 38 kDa.Can be blocked with Nudel peptide (ab39840).
Target
Function
Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL.
Tissue specificity
Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
Sequence similarities
Belongs to the nudE family.
Developmental stage
Expression peaks in mitosis.
Post-translational
modifications
Phosphorylated in mitosis. Can be phosphorylated by CDK1, CDK5 and MAPK1. Phosphorylation by CDK5 promotes interaction with KATNA1 and YWHAE.
Cellular localization
Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Localizes to the cell body of the motor neurons and colocalizes with assembled neurofilaments within axonal processes. Localizes to the microtubules of the manchette in elongated spermatids. Colocalizes with DISC1 in the perinuclear region, including the centrosome (By similarity). Localizes to the interphase centrosome and the mitotic spindle. Localizes to the kinetochore in a CENPF-dependent manner.
Target information above from: UniProt accessionQ9GZM8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- A. nidulans antibody
- DKFZp451M0318 antibody
- ENDOOLIGOPEPTIDASE A antibody
see all
Database links
- Entrez Gene: 81565 Human
- Entrez Gene: 83431 Mouse
- Omim: 607538 Human
- SwissProt: Q9GZM8 Human
- SwissProt: Q9ERR1 Mouse
- Unigene: 372123 Human
- Unigene: 31979 Mouse
Anti-Nudel antibody images:
Western blot - Nudel antibody (ab25959)
Lane 1 : Anti-Nudel antibody (ab25959) at 0.5 µg/ml
Lane 2 : Anti-Nudel antibody (ab25959) at 1 µg/ml
Lane 3 : Anti-Nudel antibody (ab25959) at 2 µg/ml
Lane 1 : Jurkat whole cell lysate.
Lane 2 : Jurkat whole cell lysate.
Lane 3 : Jurkat whole cell lysate.
Predicted band size : 38 kDa
Observed band size : 34 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - Nudel antibody (ab25959)
ab25959 at 2µg/ml staining Nudel in Jurkat cells by ICC/IF
References for Anti-Nudel antibody (ab25959)
This product has been referenced in:
- Leliveld SRet al. Insolubility of disrupted-in-schizophrenia 1 disrupts oligomer-dependent interactions with nuclear distribution element 1 and is associated with sporadic mental disease. J Neurosci 28:3839-45 (2008).Read more (PubMed: 18400883) »
- Alkam Tet al. The extensive nitration of neurofilament light chain in the hippocampus is associated with the cognitive impairment induced by amyloid beta in mice. J Pharmacol Exp Ther : (2008). WB; Mouse.Read more (PubMed: 18621989) »
See all 3 publications for this product
Publishing research using ab25959? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"