Anti-NDEL1 antibody (ab25959)
Key features and details
- Rabbit polyclonal to NDEL1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NDEL1 antibody
See all NDEL1 primary antibodies -
Description
Rabbit polyclonal to NDEL1 -
Host species
Rabbit -
Specificity
ab25959 recognises NDEL1.
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Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human NDEL1.
(Peptide available asab39840) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25959 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (1) |
Use a concentration of 2 µg/ml.
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WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 38 kDa.
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Notes |
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ICC/IF
Use a concentration of 2 µg/ml. |
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 38 kDa. |
Target
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Function
Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL. -
Tissue specificity
Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. -
Sequence similarities
Belongs to the nudE family. -
Developmental stage
Expression peaks in mitosis. -
Post-translational
modificationsPhosphorylated in mitosis. Can be phosphorylated by CDK1, CDK5 and MAPK1. Phosphorylation by CDK5 promotes interaction with KATNA1 and YWHAE. -
Cellular localization
Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Localizes to the cell body of the motor neurons and colocalizes with assembled neurofilaments within axonal processes. Localizes to the microtubules of the manchette in elongated spermatids. Colocalizes with DISC1 in the perinuclear region, including the centrosome (By similarity). Localizes to the interphase centrosome and the mitotic spindle. Localizes to the kinetochore in a CENPF-dependent manner. - Information by UniProt
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Database links
- Entrez Gene: 81565 Human
- Omim: 607538 Human
- SwissProt: Q9GZM8 Human
- Unigene: 372123 Human
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Alternative names
- A. nidulans antibody
- DKFZp451M0318 antibody
- ENDOOLIGOPEPTIDASE A antibody
see all
Images
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Lane 1 : Anti-NDEL1 antibody (ab25959) at 0.5 µg/ml
Lane 2 : Anti-NDEL1 antibody (ab25959) at 1 µg/ml
Lane 3 : Anti-NDEL1 antibody (ab25959) at 2 µg/ml
All lanes : Jurkat whole cell lysate.
Predicted band size: 38 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted? -
ab25959 at 2µg/ml staining NDEL1 in Jurkat cells by ICC/IF
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Immunofluorescence of NDEL1 in Jurkat cells using ab25959 at 10 ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab25959 has been referenced in 6 publications.
- Chapman DE et al. Regulation of in vivo dynein force production by CDK5 and 14-3-3e and KIAA0528. Nat Commun 10:228 (2019). PubMed: 30651536
- Turgay Y et al. SUN proteins facilitate the removal of membranes from chromatin during nuclear envelope breakdown. J Cell Biol 204:1099-109 (2014). Human . PubMed: 24662567
- Pei Z et al. The expression and roles of Nde1 and Ndel1 in the adult mammalian central nervous system. Neuroscience 271:119-36 (2014). PubMed: 24785679
- Leliveld SR et al. Insolubility of disrupted-in-schizophrenia 1 disrupts oligomer-dependent interactions with nuclear distribution element 1 and is associated with sporadic mental disease. J Neurosci 28:3839-45 (2008). PubMed: 18400883
- Alkam T et al. The extensive nitration of neurofilament light chain in the hippocampus is associated with the cognitive impairment induced by amyloid beta in mice. J Pharmacol Exp Ther : (2008). WB ; Mouse . PubMed: 18621989
- Li W et al. Specific developmental disruption of disrupted-in-schizophrenia-1 function results in schizophrenia-related phenotypes in mice. Proc Natl Acad Sci U S A 104:18280-5 (2007). IP ; Mouse . PubMed: 17984054