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ab39840 |
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Read our guarantee »Products:Neuroscience >> Neurology process >> Neural Signal Transduction
Anti-Nudel antibody
See all Nudel products (7) ...
Rabbit polyclonal to Nudel
ab25959 recognises Nudel.
IP, ICC/IF, WBmore details
Reacts with
Mouse, Human
Synthetic peptide (Human) - corresponding to 15 amino acids near the amino terminus of human Nudel.
Jurkat cell lysate.
Liquid
Store at +4°C.
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Neuroscience >> Neurology process >> Neural Signal Transduction
Our Abpromise guarantee covers the use of ab25959 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution. (PubMed: 17984054)
ICC/IF: Use a concentration of 2 µg/ml
WB: Use a concentration of 0.5-1.0 µg/mlPredicted molecular weight: 38 kDa.Can be blocked with Nudel peptide (ab39840).
Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL.
Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
Belongs to the nudE family.
Expression peaks in mitosis.
Phosphorylated in mitosis. Can be phosphorylated by CDK1, CDK5 and MAPK1. Phosphorylation by CDK5 promotes interaction with KATNA1 and YWHAE.
Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Localizes to the cell body of the motor neurons and colocalizes with assembled neurofilaments within axonal processes. Localizes to the microtubules of the manchette in elongated spermatids. Colocalizes with DISC1 in the perinuclear region, including the centrosome (By similarity). Localizes to the interphase centrosome and the mitotic spindle. Localizes to the kinetochore in a CENPF-dependent manner.
Target information above from: UniProt accessionQ9GZM8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - Nudel antibody (ab25959)

Lane 1 : Anti-Nudel antibody (ab25959) at 0.5 µg/ml
Lane 2 : Anti-Nudel antibody (ab25959) at 1 µg/ml
Lane 3 : Anti-Nudel antibody (ab25959) at 2 µg/ml
Lane 1 : Jurkat whole cell lysate.
Lane 2 : Jurkat whole cell lysate.
Lane 3 : Jurkat whole cell lysate.
Predicted band size : 38 kDa
Observed band size : 34 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - Nudel antibody (ab25959)

ab25959 at 2µg/ml staining Nudel in Jurkat cells by ICC/IF
This product has been referenced in:
See all 3 publications for this product
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Lane 1 : Anti-Nudel antibody (ab25959) at 0.5 µg/ml
Lane 2 : Anti-Nudel antibody (ab25959) at 1 µg/ml
Lane 3 : Anti-Nudel antibody (ab25959) at 2 µg/ml
Lane 1 : Jurkat whole cell lysate.
Lane 2 : Jurkat whole cell lysate.
Lane 3 : Jurkat whole cell lysate.
Predicted band size : 38 kDa
Observed band size : 34 kDa (why is the actual band size different from the predicted?)

ab25959 at 2µg/ml staining Nudel in Jurkat cells by ICC/IF
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