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Products:Neuroscience >> Neurology process >> Neural Signal Transduction
Overview
Product name
Anti-Nudel antibody
See all Nudel products (7) ...
Description
Goat polyclonal to Nudel
Specificity
ab81975 is expected to recognize both reported isoforms (NP_001020750.1 and NP_110435.1)
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Rabbit, Chicken, Xenopus laevis, Zebrafish, Cynomolgus Monkey, Orangutan
Immunogen
Synthetic peptide: CRNFAKDQASRKSY, corresponding to internal sequence amino acids 273 - 286 of Human Nudel (NP_001020750.1, NP_110435.1)
CRNFAKDQAS RKSY
Positive control
Human Cerebellum lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab81975 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Neuroscience >> Neurology process >> Neural Signal Transduction
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Western blot - Nudel antibody (ab81975)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab81975 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.1 - 0.3 µg/ml.Predicted molecular weight: 38 kDa.
Target
Function
Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL.
Tissue specificity
Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
Sequence similarities
Belongs to the nudE family.
Developmental stage
Expression peaks in mitosis.
Post-translational
modifications
Phosphorylated in mitosis. Can be phosphorylated by CDK1, CDK5 and MAPK1. Phosphorylation by CDK5 promotes interaction with KATNA1 and YWHAE.
Cellular localization
Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Localizes to the cell body of the motor neurons and colocalizes with assembled neurofilaments within axonal processes. Localizes to the microtubules of the manchette in elongated spermatids. Colocalizes with DISC1 in the perinuclear region, including the centrosome (By similarity). Localizes to the interphase centrosome and the mitotic spindle. Localizes to the kinetochore in a CENPF-dependent manner.
Target information above from: UniProt accessionQ9GZM8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- A. nidulans antibody
- DKFZp451M0318 antibody
- ENDOOLIGOPEPTIDASE A antibody
see all
Database links
- Entrez Gene: 417317 Chicken
- Entrez Gene: 81565 Human
- Entrez Gene: 83431 Mouse
- Entrez Gene: 100173007 Orangutan
- Entrez Gene: 100008657 Rabbit
- Entrez Gene: 170845 Rat
- Entrez Gene: 444761 Xenopus laevis
- Omim: 607538 Human
see all
Anti-Nudel antibody images:
Western blot - Nudel antibody (ab81975)
Anti-Nudel antibody (ab81975) at 0.1 µg/ml + Human Cerebellum lysate (in RIPA buffer) at 35 µg
Predicted band size : 38 kDa
Observed band size : 38 kDa
Additional bands at : 39 kDa (possible isoform).
References for Anti-Nudel antibody (ab81975)
ab81975 has not yet been referenced specifically in any publications.
Publishing research using ab81975? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"