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Products:Neuroscience >> Neurology process >> Neural Signal Transduction
Overview
Product name
Anti-Nudel (phospho T219) antibody
See all Nudel products (7) ...
Description
Rabbit polyclonal to Nudel (phospho T219)
Tested applications
ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic phosphopeptide corresponding to amino acid residues surrounding T219 of human Nudel conjugated to KLH
Positive control
Human hepatocarcinoma tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
ab60005 is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2 step procedure with the control and phosphorylated peptides. The phospho specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Neuroscience >> Neurology process >> Neural Signal Transduction
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nudel (phospho T219) antibody (ab60005)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab60005 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000ELISA: 1/1000
IHC-P: 1/50 - 1/100.
Target
Function
Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL.
Tissue specificity
Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
Sequence similarities
Belongs to the nudE family.
Developmental stage
Expression peaks in mitosis.
Post-translational
modifications
Phosphorylated in mitosis. Can be phosphorylated by CDK1, CDK5 and MAPK1. Phosphorylation by CDK5 promotes interaction with KATNA1 and YWHAE.
Cellular localization
Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Localizes to the cell body of the motor neurons and colocalizes with assembled neurofilaments within axonal processes. Localizes to the microtubules of the manchette in elongated spermatids. Colocalizes with DISC1 in the perinuclear region, including the centrosome (By similarity). Localizes to the interphase centrosome and the mitotic spindle. Localizes to the kinetochore in a CENPF-dependent manner.
Target information above from: UniProt accessionQ9GZM8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- A. nidulans antibody
- DKFZp451M0318 antibody
- ENDOOLIGOPEPTIDASE A antibody
see all
Database links
- Entrez Gene: 81565 Human
- Omim: 607538 Human
- SwissProt: Q9GZM8 Human
- Unigene: 372123 Human
Anti-Nudel (phospho T219) antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nudel (phospho T219) antibody (ab60005)
ab60005 at 1/50 dilution staining Nudel in Human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. The primary antibody was peroxidase conjugated to the secondary antibody, followed by AEC staining.
References for Anti-Nudel (phospho T219) antibody (ab60005)
ab60005 has not yet been referenced specifically in any publications.
Publishing research using ab60005? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"