Overview

  • Product nameAnti-Nup53 antibodySee all Nup53 primary antibodies ...
  • Description
    Rabbit polyclonal to Nup53
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    A synthetic peptide corresponding to a region of Human Nup53

  • Positive control
    • Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab76381 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionFunctions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs). Can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. May play a role in the association of MAD1 with the NPC.
  • Sequence similaritiesBelongs to the Nup53 family.
    Contains 1 RRM Nup35-type domain.
  • Cellular localizationNucleus > nuclear pore complex. Nucleus membrane. Tightly associated with the nuclear membrane and lamina.
  • Information by UniProt
  • Database links
  • Alternative names
    • 35 kDa nucleoporin antibody
    • Mitotic phosphoprotein 44 antibody
    • MP 44 antibody
    • MP-44 antibody
    • NP 44 antibody
    • NP44 antibody
    • Nuclear pore complex protein 53kDa antibody
    • Nuclear pore complex protein Nup 53 antibody
    • Nuclear pore complex protein Nup53 antibody
    • Nuclear pore protein NUP 53 antibody
    • Nuclear pore protein NUP53 antibody
    • Nucleoporin 35kDa antibody
    • Nucleoporin 53kDa antibody
    • Nucleoporin Nup 35 antibody
    • Nucleoporin NUP 53 antibody
    • Nucleoporin Nup35 antibody
    • Nucleoporin NUP53 antibody
    • NUP 35 antibody
    • Nup 53 antibody
    • NUP35 antibody
    • NUP53 antibody
    • NUP53_HUMAN antibody
    see all

Anti-Nup53 antibody images

  • Anti-Nup53 antibody (ab76381) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 35 kDa
    Observed band size : 31 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Nup53 antibody (ab76381)

ab76381 has not yet been referenced specifically in any publications.

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