Anti-Nup53 antibody (ab76381)

Overview

• Product nameAnti-Nup53 antibodySee all Nup53 primary antibodies ...
• Description
  Rabbit polyclonal to Nup53
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Chicken, Cow, Zebrafish
• Immunogen

  A synthetic peptide corresponding to a region of Human Nup53

• Positive controlJurkat cell lysate.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 2% Sucrose, PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Protein Trafficking
  • Nuclear Import / Export

  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • Nuclear Receptors
  • Nuclear Pore Complex

Applications

Target

• FunctionFunctions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs). Can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. May play a role in the association of MAD1 with the NPC.
• Sequence similaritiesBelongs to the Nup53 family.
  Contains 1 RRM Nup35-type domain.
• Cellular localizationNucleus > nuclear pore complex. Nucleus membrane. Tightly associated with the nuclear membrane and lamina.

Target information above from: UniProt accession Q8NFH5 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 511248 Cow
  • Entrez Gene: 129401 Human
  • Entrez Gene: 69482 Mouse
  • Entrez Gene: 295692 Rat
  • Entrez Gene: 337461 Zebrafish
  • Omim: 608140 Human
  • SwissProt: Q8NFH5 Human
  • SwissProt: Q8R4R6 Mouse

  • SwissProt: Q68FY1 Rat
  • SwissProt: Q6P6X9 Zebrafish
  • Unigene: 180591 Human
  • Unigene: 29200 Mouse
  • Unigene: 203627 Rat
  • Unigene: 5851 Zebrafish

  see all

• Alternative names
  35 kDa nucleoporin antibodyMitotic phosphoprotein 44 antibodyMP 44 antibody

  MP-44 antibodyNP 44 antibodyNP44 antibodyNuclear pore complex protein 53kDa antibodyNuclear pore complex protein Nup 53 antibodyNuclear pore complex protein Nup53 antibodyNuclear pore protein NUP 53 antibodyNuclear pore protein NUP53 antibodyNucleoporin 35kDa antibodyNucleoporin 53kDa antibodyNucleoporin Nup 35 antibodyNucleoporin NUP 53 antibodyNucleoporin Nup35 antibodyNucleoporin NUP53 antibodyNUP 35 antibodyNup 53 antibodyNUP35 antibodyNUP53 antibodyNUP53_HUMAN antibody

  see all

Anti-Nup53 antibody images

• 

  Western blot - Nup53 antibody (ab76381)
  Anti-Nup53 antibody (ab76381) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg
  
  Secondary
  HRP conjugated anti-Rabbit IgG at 1/50000 dilution
  
  Predicted band size : 35 kDa
  Observed band size : 31 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.