Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Overview
Product name
Anti-Nurr1 antibody
See all Nurr1 products (10) ...
Description
Rabbit polyclonal to Nurr1
Specificity
ab12261 is a new Nurr1 affinity purified antibody that is shown to work in WB (see image). The previous affinity purified antibody associated with this ab number (ab12261) was unpublished based on a negative review.
Tested applications
Cross reactivity
Reacts with
Rat, Human
Predicted to work with
Mouse
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Nurr1.
(Peptide available as ab12301.)
Positive control
This antibody gave a positive signal in the following lysates: Rat Hippocampus Tissue; Hela Whole Cell; Jurkat Whole Cell; A431 Whole Cell.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Neurogenesis
Immunology >> Immune System Diseases >> Autoimmune
Neuroscience >> Neurology process >> Neural Signal Transduction
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
-
Western blot - Nurr1 antibody (ab12261)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab12261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 66.5 kDa (predicted molecular weight: 66.5 kDa).
Target
Function
Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons.
Tissue specificity
Expressed in a number of cell lines of T-cell, B-cell and fibroblast origin. Strong expression in brain tissue.
Sequence similarities
Belongs to the nuclear hormone receptor family. NR4 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
Developmental stage
Rapidly and only very transiently expressed after cell activation, during the G0-G1 transition of the cell cycle.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP43354
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- HZF 3 antibody
- HZF3 antibody
- Immediate-early response protein NOT antibody
see all
Database links
- Entrez Gene: 4929 Human
- Entrez Gene: 18227 Mouse
- Entrez Gene: 54278 Rat
- Omim: 601828 Human
- SwissProt: P43354 Human
- SwissProt: Q06219 Mouse
- SwissProt: Q07917 Rat
- SwissProt: Q3LZI9 Rat
see all
Anti-Nurr1 antibody images:
Western blot - Nurr1 antibody (ab12261)
All lanes : Anti-Nurr1 antibody (ab12261) at 1 µg/ml
Lane 1 : Hela Lysate
Lane 2 : Jurkat Lysate
Lane 3 : A431 Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 66.5 kDa
Observed band size : 66.5 kDa
References for Anti-Nurr1 antibody (ab12261)
ab12261 has not yet been referenced specifically in any publications.
Publishing research using ab12261? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"