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Overview

Product name

Anti-OA1 antibody
See all OA1 products (4) ...

Description

Rabbit polyclonal to OA1

Specificity

Specific for OA1

Tested applications

ICC/IF, WB, IHC-P, IHC-Frmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide as a part of human ocular albinism type 1 protein (G protein-coupled receptor 143 or GPR143, OA1) conjugated to immunogenic carrier protein

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: Whole serum

Purity

Whole antiserum

Clonality

Polyclonal

Isotype

IgG

  • Western blot - Anti-OA1 antibody (ab65635)Western blot - Anti-OA1 antibody (ab65635) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab65635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide

    ICC/IF

     ICC/IF: Use at an assay ...Read more →

    ICC/IF: Use at an assay dependent concentration. (PubMed: 21730137)

  • ShowHide1 Image

    WB

     WB: 1/300 - 1/4000.Predi...Read more →

    WB: 1/300 - 1/4000.Predicted molecular weight: 44 kDa.

  • IHC-P

     IHC-P: 1/300 - 1/4000.

    IHC-P: 1/300 - 1/4000.

  • IHC-Fr

     IHC-Fr: 1/300 - 1/4000.

    IHC-Fr: 1/300 - 1/4000.

Target

Function

Receptor for tyrosine, L-DOPA and dopamine. After binding to L-DOPA, stimulates Ca(2+) influx into the cytoplasm, increases secretion of the neurotrophic factor SERPINF1 and relocalizes beta arrestin at the plasma membrane; this ligand-dependent signaling occurs through a G(q)-mediated pathway in melanocytic cells. Its activity is mediated by G proteins which activate the phosphoinositide signaling pathway. Plays also a role as an intracellular G protein-coupled receptor involved in melanosome biogenesis, organization and transport.

Tissue specificity

Expressed at high levels in the retina, including the retinal pigment epithelium (RPE), and in melanocytes. Weak expression is observed in brain and adrenal gland.

Involvement in disease

Defects in GPR143 are the cause of albinism ocular type 1 (OA1) [MIM:300500]; also known as Nettleship-Falls type ocular albinism. Form of albinism affecting only the eye. Pigment of the hair and skin is normal or only slightly diluted. Eyes may be severely affected with photophobia and reduced visual acuity. Nystagmus or strabismus are often associated. The irides and fundus are depigmented.
Defects in GPR143 are the cause of Nystagmus congenital X-linked type 6 (NYS6) [MIM:300814]. It is a condition defined as conjugated, spontaneous and involuntary ocular oscillations that appear at birth or during the first three months of life. Other associated features may include mildly decreased visual acuity, strabismus, astigmatism, and occasionally head nodding.

Sequence similarities

Belongs to the G-protein coupled receptor OA family.

Domain

The cytoplasmic domain 3 and the C-terminus tail domain contain the lysosomal sorting signals and are necessary and sufficient for intracellular retention and delivery to lysosomal and melanosomal, respectively in melanocytic and non-melanocytic cells.

Post-translational
modifications

Glycosylated.
Phosphorylated.

Cellular localization

Golgi apparatus. Melanosome membrane. Lysosome membrane. Apical cell membrane. Distributed throughout the endo-melanosomal system but most of endogenous protein is localized in unpigmented stage II melanosomes. Its expression on the apical cell membrane is sensitive to tyrosine.

Target information above from: UniProt accessionP51810 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • ALBINISM OCULAR TYPE I antibody
  • G protein coupled receptor 143 antibody
  • G-protein coupled receptor 143 antibody
  • GP143_HUMAN antibody
  • GPR143 antibody
  • MOA1 antibody
  • NETTLESHIP FALLS TYPE OCULAR ALBINISM antibody
  • Ocular albinism type 1 protein antibody
  • Ocular albinism type 1 protein homolog antibody
  • Ocular albinism1 Nettleship Falls type antibody
see all

Anti-OA1 antibody images:

  Western blot - Anti-OA1 antibody (ab65635)

Western blot - Anti-OA1 antibody (ab65635)



Predicted band size : 44 kDa


A) Western blot of proteins from human RPE immunoprecipitated with ab65635. SDS–PAGE was followed by incubation with anti-Gαi3 antibodies. Left lane: RPE extract incubated with ab65635; right lane: RPE extract incubated with pre-immune serum.

B) Western blot analysis of the proteins from human RPE immunoprecipitated with anti-Gαi3 antibody. SDS–PAGE was followed by incubation with ab65635. Left lane: RPE extract incubated with Gαi3 antibody; right lane: RPE extract incubated with pre-immune serum. Two isoforms of OA1 have apparent molecular weights of 45 and 48 kDa (arrowheads).

Image from Young A et al, PLoS One. 2011;6(9):e24376. Epub 2011 Sep 8, Fig 5. DOI 10.1371/journal.pone.0024376

References for Anti-OA1 antibody (ab65635)

This product has been referenced in:

  • Giordano Fet al. The ocular albinism type 1 (OA1) GPCR is ubiquitinated and its traffic requires endosomal sorting complex responsible for transport (ESCRT) function. Proc Natl Acad Sci U S A 108:11906-11 (2011). ICC/IF; Human.Read more (PubMed: 21730137) »
  • Young Aet al. Specific interaction of gai3 with the oa1 g-protein coupled receptor controls the size and density of melanosomes in retinal pigment epithelium. PLoS One 6:e24376 (2011). WB; Human.Read more (PubMed: 21931697) »

See all 2 publications for this product

Publishing research using ab65635? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"