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Products:Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> More GPCR
Overview
Product name
Anti-OA1 antibody
See all OA1 products (4) ...
Description
Rabbit polyclonal to OA1
Specificity
Specific for OA1
Tested applications
ICC/IF, WB, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide as a part of human ocular albinism type 1 protein (G protein-coupled receptor 143 or GPR143, OA1) conjugated to immunogenic carrier protein
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Sensory System >> Somatosensory system >> Touch
Neuroscience >> Sensory System >> Visual system
Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> More GPCR
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Western blot - Anti-OA1 antibody (ab65635)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab65635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent concentration. (PubMed: 21730137)
WB: 1/300 - 1/4000.Predicted molecular weight: 44 kDa.
IHC-P
IHC-P: 1/300 - 1/4000.IHC-P: 1/300 - 1/4000.
IHC-Fr
IHC-Fr: 1/300 - 1/4000.IHC-Fr: 1/300 - 1/4000.
Target
Function
Receptor for tyrosine, L-DOPA and dopamine. After binding to L-DOPA, stimulates Ca(2+) influx into the cytoplasm, increases secretion of the neurotrophic factor SERPINF1 and relocalizes beta arrestin at the plasma membrane; this ligand-dependent signaling occurs through a G(q)-mediated pathway in melanocytic cells. Its activity is mediated by G proteins which activate the phosphoinositide signaling pathway. Plays also a role as an intracellular G protein-coupled receptor involved in melanosome biogenesis, organization and transport.
Tissue specificity
Expressed at high levels in the retina, including the retinal pigment epithelium (RPE), and in melanocytes. Weak expression is observed in brain and adrenal gland.
Involvement in disease
Defects in GPR143 are the cause of albinism ocular type 1 (OA1) [MIM:300500]; also known as Nettleship-Falls type ocular albinism. Form of albinism affecting only the eye. Pigment of the hair and skin is normal or only slightly diluted. Eyes may be severely affected with photophobia and reduced visual acuity. Nystagmus or strabismus are often associated. The irides and fundus are depigmented.
Defects in GPR143 are the cause of Nystagmus congenital X-linked type 6 (NYS6) [MIM:300814]. It is a condition defined as conjugated, spontaneous and involuntary ocular oscillations that appear at birth or during the first three months of life. Other associated features may include mildly decreased visual acuity, strabismus, astigmatism, and occasionally head nodding.
Sequence similarities
Belongs to the G-protein coupled receptor OA family.
Domain
The cytoplasmic domain 3 and the C-terminus tail domain contain the lysosomal sorting signals and are necessary and sufficient for intracellular retention and delivery to lysosomal and melanosomal, respectively in melanocytic and non-melanocytic cells.
Post-translational
modifications
Glycosylated.
Phosphorylated.
Cellular localization
Golgi apparatus. Melanosome membrane. Lysosome membrane. Apical cell membrane. Distributed throughout the endo-melanosomal system but most of endogenous protein is localized in unpigmented stage II melanosomes. Its expression on the apical cell membrane is sensitive to tyrosine.
Target information above from: UniProt accessionP51810
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ALBINISM OCULAR TYPE I antibody
- G protein coupled receptor 143 antibody
- G-protein coupled receptor 143 antibody
see all
Database links
- Entrez Gene: 4935 Human
- Omim: 300500 Human
- SwissProt: P51810 Human
- Unigene: 74124 Human
Anti-OA1 antibody images:
Western blot - Anti-OA1 antibody (ab65635)
Predicted band size : 44 kDa
A) Western blot of proteins from human RPE immunoprecipitated with ab65635. SDS–PAGE was followed by incubation with anti-Gαi3 antibodies. Left lane: RPE extract incubated with ab65635; right lane: RPE extract incubated with pre-immune serum.B) Western blot analysis of the proteins from human RPE immunoprecipitated with anti-Gαi3 antibody. SDS–PAGE was followed by incubation with ab65635. Left lane: RPE extract incubated with Gαi3 antibody; right lane: RPE extract incubated with pre-immune serum. Two isoforms of OA1 have apparent molecular weights of 45 and 48 kDa (arrowheads).
Image from Young A et al, PLoS One. 2011;6(9):e24376. Epub 2011 Sep 8, Fig 5. DOI 10.1371/journal.pone.0024376
References for Anti-OA1 antibody (ab65635)
This product has been referenced in:
- Giordano Fet al. The ocular albinism type 1 (OA1) GPCR is ubiquitinated and its traffic requires endosomal sorting complex responsible for transport (ESCRT) function. Proc Natl Acad Sci U S A 108:11906-11 (2011). ICC/IF; Human.Read more (PubMed: 21730137) »
- Young Aet al. Specific interaction of gai3 with the oa1 g-protein coupled receptor controls the size and density of melanosomes in retinal pigment epithelium. PLoS One 6:e24376 (2011). WB; Human.Read more (PubMed: 21931697) »
See all 2 publications for this product
Publishing research using ab65635? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"