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Anti-OA1 antibody (ab65637)

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Overview

Product name

Anti-OA1 antibody
See all OA1 products (4) ...

Description

Rabbit polyclonal to OA1

Specificity

Specific for OA1

Tested applications

WB, IHC-P, IHC-Frmore details

Cross reactivity

Reacts with

Mouse

Immunogen

Synthetic peptide as a part of mouse ocular albinism type 1 protein homolog (G-protein coupled receptor 143 or GPR143, OA1) conjugated to immunogenic carrier protein.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: Whole serum

Purity

Whole antiserum

Clonality

Polyclonal

Isotype

IgG

Research areas

Neuroscience >> Sensory System >> Somatosensory system >> Touch
Neuroscience >> Sensory System >> Visual system
Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> More GPCR

Applications

Show applications key

Our Abpromise guarantee covers the use of ab65637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • WB

     

  • IHC-P

     

  • IHC-Fr

     

Application notes

IHC-P: 1/300 - 1/4000. Antigen retrieval is recommended.
IHC-Fr: 1/300 - 1/4000.
WB: 1/300 - 1/4000. Predicted molecular weight: 44 kDa. This antibody was originally tested by truncated recombinant protein for WB, no data avaiable yet on endogenous protein.




Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Receptor for tyrosine, L-DOPA and dopamine. After binding to L-DOPA, stimulates Ca(2+) influx into the cytoplasm, increases secretion of the neurotrophic factor SERPINF1 and relocalizes beta arrestin at the plasma membrane; this ligand-dependent signaling occurs through a G(q)-mediated pathway in melanocytic cells. Its activity is mediated by G proteins which activate the phosphoinositide signaling pathway. Plays also a role as an intracellular G protein-coupled receptor involved in melanosome biogenesis, organization and transport.

Tissue specificity

Expressed at high levels in the retina, including the retinal pigment epithelium (RPE), and in melanocytes. Weak expression is observed in brain and adrenal gland.

Involvement in disease

Defects in GPR143 are the cause of albinism ocular type 1 (OA1) [MIM:300500]; also known as Nettleship-Falls type ocular albinism. Form of albinism affecting only the eye. Pigment of the hair and skin is normal or only slightly diluted. Eyes may be severely affected with photophobia and reduced visual acuity. Nystagmus or strabismus are often associated. The irides and fundus are depigmented.
Defects in GPR143 are the cause of Nystagmus congenital X-linked type 6 (NYS6) [MIM:300814]. It is a condition defined as conjugated, spontaneous and involuntary ocular oscillations that appear at birth or during the first three months of life. Other associated features may include mildly decreased visual acuity, strabismus, astigmatism, and occasionally head nodding.

Sequence similarities

Belongs to the G-protein coupled receptor OA family.

Domain

The cytoplasmic domain 3 and the C-terminus tail domain contain the lysosomal sorting signals and are necessary and sufficient for intracellular retention and delivery to lysosomal and melanosomal, respectively in melanocytic and non-melanocytic cells.

Post-translational
modifications

Glycosylated.
Phosphorylated.

Cellular localization

Golgi apparatus. Melanosome membrane. Lysosome membrane. Apical cell membrane. Distributed throughout the endo-melanosomal system but most of endogenous protein is localized in unpigmented stage II melanosomes. Its expression on the apical cell membrane is sensitive to tyrosine.

Target information above from: UniProt accessionP51810 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • ALBINISM OCULAR TYPE I antibody
  • G protein coupled receptor 143 antibody
  • G-protein coupled receptor 143 antibody
  • GP143_HUMAN antibody
  • GPR143 antibody
  • MOA1 antibody
  • NETTLESHIP FALLS TYPE OCULAR ALBINISM antibody
  • Ocular albinism type 1 protein antibody
  • Ocular albinism type 1 protein homolog antibody
  • Ocular albinism1 Nettleship Falls type antibody
see all

Database links

References for Anti-OA1 antibody (ab65637)

ab65637 has not yet been referenced specifically in any publications.

Publishing research using ab65637? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"