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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Overview
Product name
Anti-OAS1 antibody
See all OAS1 products (4) ...
Description
Mouse monoclonal to OAS1
Tested applications
IHC-P, ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: SVSRRDKSKQ VWEAVLLPLS LLSMMDLRNT PAKSLDKFIE DYLLPDTCFR MQINHAIDII CGFLKERCFR GSSYPVCVSK VVKGGSSGKG TTLRGRSDAD , corresponding to amino acids 1-100 of Human OAS1
SVSRRDKSKQ VWEAVLLPLS LLSMMDLRNT PAKSLDKFIE DYLLPDTCFR MQINHAIDII CGFLKERCFR GSSYPVCVSK VVKGGSSGKG TTLRGRSDAD
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab55771 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 3 µg/ml
ICC/IF: Use a concentration of 10 µg/ml
WB: Use a concentration of 1 µg/mlDetects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
Target
Function
May play a role in mediating resistance to virus infection, control of cell growth, differentiation, and apoptosis.
Sequence similarities
Belongs to the 2-5A synthase family.
Cellular localization
Mitochondrion. Nucleus. Microsome. Endoplasmic reticulum. Associated with different subcellular fractions such as mitochondrial, nuclear, and rough/smooth microsomal fractions.
Target information above from: UniProt accessionP00973
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- (2 5')oligo(A) synthetase 1 antibody
- (2-5'')oligo(A) synthase 1 antibody
- 2 5 Oligoadenylate Synthetase 1 antibody
see all
Database links
- Entrez Gene: 4938 Human
- Omim: 164350 Human
- SwissProt: P00973 Human
- Unigene: 524760 Human
Anti-OAS1 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - OAS1 antibody (ab55771)
OAS1 antibody (ab55771) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human colon.
Western blot - OAS1 antibody (ab55771)
All lanes : Anti-OAS1 antibody (ab55771) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 46 kDa
Observed band size : 46 kDa
Additional bands at : 36 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 16 minutes
Immunocytochemistry/ Immunofluorescence-OAS1 antibody(ab55771)
ICC/IF image of ab55771 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55771, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-OAS1 antibody (ab55771)
ab55771 has not yet been referenced specifically in any publications.
Publishing research using ab55771? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"