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Products:Immunology >> Innate Immunity >> Cytokines >> Interferons
Overview
Product name
Anti-OASL antibody - Carboxyterminal end
See all OASL products (2) ...
Description
Rabbit polyclonal to OASL - Carboxyterminal end
Specificity
This antibody is specific for OASL (C term).
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
A KLH conjugated synthetic peptide (10-30 aa in length) at the C-term of last 50 aa of human OASL.
Positive control
HL60 lysate and breast carcinoma tissue
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Other Antibodies >> Other Antibodies
Immunology >> Innate Immunity >> Cytokines >> Interferons
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38325 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/100 - 1/500.Detects a band of approximately 60 kDa (predicted molecular weight: 59 kDa).
ELISA
ELISA: 1/1000ELISA: 1/1000
IHC-P: 1/50 - 1/100.
Target
Relevance
2-prime,5-prime oligoadenylates (2-5As) bind to and activate RNase L, leading to degradation of RNA and inhibition of protein synthesis. 2-5As are produced by 2-5A synthetases (OASs), a highly-conserved family of interferon-induced enzymes. The predicted 514-amino acid human p59OASL (2-5A synthetases-like) protein shares a highly conserved N-terminal domain with other OASs. The C-terminal portion of p59OASL contains 2 ubiquitin-like domains. p59OASL is expressed in most tissues, with the highest levels in hematopoietic tissues, colon, and stomach.
Cellular localization
Cytoplasmic and Nuclear
Alternative names
- p59 OASL antibody
- 2' 5' oligoadenylate synthetase like antibody
- 2'-5' oligoadenylate synthetase-like 2 antibody
see all
Database links
- Entrez Gene: 8638 Human
- Omim: 603281 Human
- SwissProt: Q15646 Human
- SwissProt: Q17R95 Human
- Unigene: 118633 Human
Anti-OASL antibody - Carboxyterminal end images:
Western blot - OASL antibody (ab38325)
Anti-OASL antibody - Carboxyterminal end (ab38325) at 1/100 dilution + HL60 lysate
Predicted band size : 59 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Additional bands at : 37 kDa,70 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - OASL antibody (ab38325)
Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with ab38325 at a 1/50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
References for Anti-OASL antibody - Carboxyterminal end (ab38325)
ab38325 has not yet been referenced specifically in any publications.
Publishing research using ab38325? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"