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Western blot - OASL antibody (ab38325)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - OASL antibody (ab38325)
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Product Name
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OASL antibody - Carboxyterminal end
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See all OASL antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to OASL - Carboxyterminal end
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Immunogen
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A KLH conjugated synthetic peptide (10-30 aa in length) at the C-term of last 50 aa of human OASL.
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Reacts with
(species key)
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Hu
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Specificity
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This antibody is specific for OASL (C term).
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/1000.
IHC-P: 1/50 - 1/100.
WB: 1/100 - 1/500. Detects a band of approximately 60 kDa (predicted molecular weight: 59 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HL60 lysate and breast carcinoma tissue
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Cell Biology >> Other Antibodies >> Other Antibodies
Immunology >> Innate Immunity >> Cytokines >> Interferons
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Relevance
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2-prime,5-prime oligoadenylates (2-5As) bind to and activate RNase L, leading to degradation of RNA and inhibition of protein synthesis. 2-5As are produced by 2-5A synthetases (OASs), a highly-conserved family of interferon-induced enzymes. The predicted 514-amino acid human p59OASL (2-5A synthetases-like) protein shares a highly conserved N-terminal domain with other OASs. The C-terminal portion of p59OASL contains 2 ubiquitin-like domains. p59OASL is expressed in most tissues, with the highest levels in hematopoietic tissues, colon, and stomach.
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OASL antibody - Carboxyterminal end (ab38325) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.250 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this OASL antibody - Carboxyterminal end is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for OASL antibody - Carboxyterminal end images, references, products related to ab38325 and other tools.
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OASL antibody - Carboxyterminal end images:
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 Western blot - OASL antibody (ab38325)
OASL antibody - Carboxyterminal end (ab38325) at 1/100 dilution + HL60 lysate
Predicted band size : 59 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Additional bands at : 37 kDa,70 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - OASL antibody (ab38325)
Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with ab38325 at a 1/50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Search PubMed (MEDLINE) for references to OASL
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OASL antibody - Carboxyterminal end - more information
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Customer reviews (feedback) regarding OASL antibody - Carboxyterminal end |
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OASL antibody - Carboxyterminal end - related products:
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OASL antibody - Carboxyterminal end - other tools:
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Contact Abcam with a Technical Enquiry about OASL antibody - Carboxyterminal end |
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