The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 59 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionDoes not have 2'-5'-OAS activity, but can bind double-stranded RNA. Displays antiviral activity against encephalomyocarditis virus (EMCV) and hepatitis C virus (HCV) via an alternative antiviral pathway independent of RNase L.
Tissue specificityExpressed in most tissues, with the highest levels in primary blood Leukocytes and other hematopoietic system tissues, colon, stomach and to some extent in testis.
Sequence similaritiesBelongs to the 2-5A synthase family. Contains 2 ubiquitin-like domains.
DomainThe ubiquitin-like domains are essential for its antiviral activity.
Cellular localizationCytoplasm and Nucleus > nucleolus. Cytoplasm.