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Overview

  • Product nameAnti-OATP2 antibodySee all OATP2 primary antibodies ...
  • Description
    Rabbit polyclonal to OATP2
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 273-302 of Human OATP2 (Q9Y6L6)

  • Positive controlHepG2 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab103065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/100 - 1/500. Predicted molecular weight: 76 kDa.

Target

  • FunctionMediates the Na(+)-independent uptake of organic anions such as pravastatin, taurocholate, methotrexate, dehydroepiandrosterone sulfate, 17-beta-glucuronosyl estradiol, estrone sulfate, prostaglandin E2, thromboxane B2, leukotriene C3, leukotriene E4, thyroxine and triiodothyronine. May play an important role in the clearance of bile acids and organic anions from the liver.
  • Tissue specificityHighly expressed in liver. Not detected in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocyte.
  • Sequence similaritiesBelongs to the organo anion transporter (TC 2.A.60) family.
    Contains 1 Kazal-like domain.
  • Cellular localizationBasolateral cell membrane. Detected in basolateral membranes of hepatocytes.

    • Target information above from: UniProt accession Q9Y6L6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Liver-specific organic anion transporter 1 antibodyLST-1 antibodyLST1 antibody
        OATP-2 antibodyOATP-C antibodyOATP1B1 antibodyOATPC antibodySLC21A6 antibodySLCO1B1 antibodySO1B1_HUMAN antibodySodium-independent organic anion-transporting polypeptide 2 antibodySolute carrier family 21 member 6 antibodySolute carrier organic anion transporter family member 1B1 antibody
      see all

    Anti-OATP2 antibody images

    • Western blot - OATP2 antibody (ab103065)
      Western blot - OATP2 antibody (ab103065)
      Anti-OATP2 antibody (ab103065) at 1/100 dilution + HepG2 cell lysate at 35 µg

      Predicted band size : 76 kDa

    References for Anti-OATP2 antibody (ab103065)

    ab103065 has not yet been referenced specifically in any publications.

    Publishing research using ab103065 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab103065 Submit a Question for ab103065

    Displaying 1 - 6 of 6 results for Abreviews and Q&A

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    Do you have any information about whether or not the Anti-OATP2 antibody (ab103065) cross-reacts with rat/mouse? We are interested in this protein in rat and mouse and were wondering if this antibody could work?

    Thank you for contacting Abcam.

    I blasted the immunogen sequence for ab103065, to see what the level of homology would be with mouse and rat would be, and for both the level of homogly is below 70%. Therefore I would not feel confident in recomme...

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    Hi xxxxxx,

    Our customer is happy to receive a free replacement of AB15441.

    Could you please organise this to be sent out to us ASAP.

    Kind regards,

    Thank you for kindly confirming these details as this enables us to closely monitor the quality of our products

    We would be pleased to arrange a replacement product free of charge. I am forwarding these details on to our Distributor’s Tea...

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    Hi Karin,

    Our customer has responded with the following;

    I am sorry for the delay in reply. I am trying to find out the differences between the two antibodies and what is best suited for my application (ie for use in western blots to...

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    Thank you for contacting us again.

    I am sorry that your customer has been a bit confused by the situation. I have been in close contact with the source of ab103065. They have retested the lot which your customer has been using and consistently g...

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    Hi,

    Hopefully you’ll be able to follow this up from the email trail below.

    In summary, the customer has already filled in a questionnaire which xxx sent to you, they applied your suggested changes to the WB protocol without suc...

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    Thank you for following up on this complaint.


    I have looked into the data again that the customer has shared with us and it seems that the antibody is picking up a non-specific band when the UGT1A1 is expressed. I understand that your cust...

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    Hi,

    My customer has come back with the following:

    I followed the steps that you suggested but had no luck so far. Unfortunately I haven't got any more antibody left .

    I attached a pic of the latest WB (with the over night ...

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    I am currently still looking into the most appropriate replacement and controls to suggest for your customer. I will get back to you with this as soon as possible. I am very sorry for the delay and the inconvenience this is causing.

    I was hopin...

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    LOT NUMBER GR38171-1 ORDER NUMBER xxxxx DESCRIPTION OF THE PROBLEM Observed high background Bands in negative control SAMPLE Recombinant OATP1B1 expressed in MDCK11 cells –used cell lysates PRIMARY ANTIBODY 1/100 in 3% skim milk/TBST for 2 hours was...

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    Thank you for contacting us and reporting the problems you have experienced in using the anti-OATP2 antibody (ab103065). The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"