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All lanes : Anti-OBFC2B antibody (ab68812) at 1/500 dilution
Lane 1 : OBFC2B transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 23 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
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